Cryo-electron microscopy (cryo-EM) is rapidly becoming an attractive method in the field of
structural biology. With the exploding popularity of cryo-EM, sample preparation must evolve …

All steps of cryogenic electron-microscopy (cryo-EM) workflows have rapidly evolved over
the last decade. Advances in both single-particle analysis (SPA) cryo-EM and cryo-electron …

Recent events have pushed RNA research into the spotlight. Continued discoveries of RNA
with unexpected diverse functions in healthy and diseased cells, such as the role of RNA as …

Summary mRNA function is influenced by modifications that modulate canonical nucleobase
behavior. We show that a single modification mediates distinct impacts on mRNA translation …

Proteasomal degradation of ubiquitylated proteins is tightly regulated at multiple levels,–. A
primary regulatory checkpoint is the removal of ubiquitin chains from substrates by the …

Single-particle cryogenic electron microscopy (cryo-EM) allows reconstruction of high-
resolution structures of proteins in different conformations. Protein function often involves …

To advance microfluidic integration, we present the use of two-photon additive
manufacturing to fold 2D channel layouts into compact free-form 3D fluidic circuits with …

Translation initiation defines the identity and quantity of a synthesized protein. The process
is dysregulated in many human diseases,. A key commitment step is when the ribosomal …

We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study
short-lived molecular states. Using piezoelectric dispensing, two independent streams of …

During translation, a conserved GTPase elongation factor—EF-G in bacteria or eEF2 in
eukaryotes—translocates tRNA and mRNA through the ribosome. EF-G has been proposed …