Ever since the inception of light microscopy, the laws of physics have seemingly thwarted
every attempt to visualize the processes of life at its most fundamental, sub-cellular, level …

The first reporter systems were developed in the early 1980s and were based on measuring
the activity of an enzyme—as a surrogate measure of promoter-driven transcriptional activity …

Although ubiquitous in biological studies, the enhanced green and yellow fluorescent
proteins (EGFP and EYFP) were not specifically optimized for neuroscience, and their …

Lysozyme with a small monomeric globular enzymatic protein is part of the innate immune
system, and its deficiency can cause the increased incidence of disease. Herein, we devise …

Methane monooxygenase (MMO), which exists in particulate (pMMO) or soluble forms
(sMMO) in methanotrophic bacteria, is an industrially promising enzyme that catalyses …

Single-molecule localization microscopy techniques have proven to be essential tools for
quantitatively monitoring biological processes at unprecedented spatial resolution …

Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for
optical nanoscopy because they make it possible to image with lower light doses. However …

Genetically-encoded biosensors based on a single fluorescent protein are widely used to
visualize analyte levels or enzymatic activities in cells, though usually to monitor relative …

Advances in the development of small photoblinking semiconducting polymer dots (Pdots)
have attracted great interest for use in super-resolution microscopy. However, multicolor …

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging
sub-wavelength-sized objects, leaving its mark in multiple branches of biology and …