Hanging drop sample preparation improves sensitivity of spatial proteomics

Y Kwon, PD Piehowski, R Zhao, RL Sontag, RJ Moore… - Lab on a Chip, 2022 - pubs.rsc.org
Spatial proteomics holds great promise for revealing tissue heterogeneity in both
physiological and pathological conditions. However, one significant limitation of most spatial …

Kinetics of insulin crystal nucleation, energy barrier, and nucleus size

CN Nanev, FV Hodzhaoglu, IL Dimitrov - Crystal growth & design, 2011 - ACS Publications
Number density of insulin crystals versus nucleation time dependences were measured
simultaneously, during the same experiment, at four typical places: in solution bulk, at the …

Analysis of oscillatory rocking curve by dynamical diffraction in protein crystals

R Suzuki, H Koizumi, K Hirano… - Proceedings of the …, 2018 - National Acad Sciences
High-quality protein crystals meant for structural analysis by X-ray diffraction have been
grown by various methods. The observation of dynamical diffraction in protein crystals is an …

Gold nanoparticles induce protein crystallization

F Hodzhaoglu, F Kurniawan, V Mirsky… - Crystal Research and …, 2008 - Wiley Online Library
Nucleation of protein crystals by gold nanoparticles was observed. Lysozyme and ferritin
were used as model proteins. The effect was established with uncoated gold nanoparticles …

Hardness and slip systems of orthorhombic hen egg-white lysozyme crystals

R Suzuki, T Kishi, S Tsukashima… - Philosophical …, 2016 - Taylor & Francis
Hardness and slip systems by an indentation method were investigated on different habit
planes of orthorhombic hen egg-white lysozyme (O-HEWL) crystals containing water. A …

Direct observation of stress-induced dislocations in protein crystals by synchrotron X-ray topography

R Suzuki, M Tachibana, H Koizumi, K Kojima - Acta Materialia, 2018 - Elsevier
Stress-induced dislocations in glucose isomerase (GI) crystals were investigated by
synchrotron X-ray topography with simple indentation method. It is observed that the …

A systematic comparison of sitting and hanging-drop crystallization using traditional and cross-diffusion microbatch crystallization plates

H Hou, M Shi, SY Hu, F Ahmad, B Zhang… - Journal of Crystal …, 2019 - Elsevier
Obtaining good quality protein crystals is still desirable for high resolution structural
determination of the proteins using crystallography. The crystal quality is affected by the …

Kinetics and intimate mechanism of protein crystal nucleation

CN Nanev - Progress in crystal growth and characterization of …, 2013 - Elsevier
Experimental and theoretical investigations on protein crystal nucleation are reviewed.
Various experimental applications of the classical principle, which requires separation of the …

Microindentation hardness of protein crystals under controlled relative humidity

T Kishi, R Suzuki, C Shigemoto, H Murata, K Kojima… - Crystals, 2017 - mdpi.com
Vickers microindentation hardness of protein crystals was investigated on the (110) habit
plane of tetragonal hen egg-white lysozyme crystals containing intracrystalline water at …

Brittleness of protein crystals

CN Nanev - Crystal Research and Technology, 2012 - Wiley Online Library
A simple technique for studying the brittleness of small crystals is reported. The limits of
fracture toughness of tetragonal hen‐egg white lysozyme crystals, oriented with their c‐axis …