Transient gene expression is an effective experimental tool for the research into the fine mechanisms of plant gene function: advantages, limitations, and solutions

AA Tyurin, AV Suhorukova, KV Kabardaeva… - Plants, 2020 - mdpi.com
A large data array on plant gene expression accumulated thanks to comparative omic
studies directs the efforts of researchers to the specific or fine effects of the target gene …

Agroinfiltration mediated scalable transient gene expression in genome edited crop plants

M Kaur, P Manchanda, A Kalia, FK Ahmed… - International Journal of …, 2021 - mdpi.com
Agrobacterium-mediated transformation is one of the most commonly used genetic
transformation method that involves transfer of foreign genes into target plants …

Pollen magnetofection for genetic modification with magnetic nanoparticles as gene carriers

X Zhao, Z Meng, Y Wang, W Chen, C Sun, B Cui, J Cui… - Nature plants, 2017 - nature.com
Genetic modification plays a vital role in breeding new crops with excellent traits. Almost all
the current genetic modification methods require regeneration from tissue culture, involving …

The plant unique ESCRT component FREE1 regulates autophagosome closure

Y Zeng, B Li, S Huang, H Li, W Cao, Y Chen… - Nature …, 2023 - nature.com
The energy sensor AMP-activated protein kinase (AMPK) can activate autophagy when
cellular energy production becomes compromised. However, the degree to which nutrient …

Sheet-like clay nanoparticles deliver RNA into developing pollen to efficiently silence a target gene

J Yong, R Zhang, S Bi, P Li, L Sun, N Mitter… - Plant …, 2021 - academic.oup.com
Topical application of double-stranded RNA (dsRNA) can induce RNA interference (RNAi)
and modify traits in plants without genetic modification. However, delivering dsRNA into …

Autophagy and its mediated mitochondrial quality control maintain pollen tube growth and male fertility in Arabidopsis

H Yan, M Zhuang, X Xu, S Li, M Yang, N Li, X Du… - Autophagy, 2023 - Taylor & Francis
Macroautophagy/autophagy, a major catabolic pathway in eukaryotes, participates in plant
sexual reproduction including the processes of male gametogenesis and the self …

Nanocarriers for transgene expression in pollen as a plant biotechnology tool

TTS Lew, M Park, Y Wang, P Gordiichuk… - ACS Materials …, 2020 - ACS Publications
As the male gametophyte of flowering plants, pollen grains are ideal targets for the
introduction of transgenes for plant genetic engineering and crop improvement. However …

Trans-Golgi Network-Located AP1 Gamma Adaptins Mediate Dileucine Motif-Directed Vacuolar Targeting in Arabidopsis

X Wang, Y Cai, H Wang, Y Zeng, X Zhuang, B Li… - The Plant …, 2014 - academic.oup.com
Membrane proteins on the tonoplast are indispensible for vacuolar functions in plants.
However, how these proteins are transported to the vacuole and how they become …

Isolation, culture, and transient transformation of plant protoplasts

J Shen, J Fu, J Ma, X Wang, C Gao… - Current protocols in …, 2014 - Wiley Online Library
Transient gene expression in protoplasts, which has been used in several plant species, is
an important and versatile tool for rapid functional gene analysis, protein subcellular …

No evidence for transient transformation via pollen magnetofection in several monocot species

Z Vejlupkova, C Warman, R Sharma, HV Scheller… - Nature plants, 2020 - nature.com
MATTERS ARISING NATURE PLANTS trials indicates a statistically significant difference
between the positive control biolistic method and both the negative control and the …