[HTML][HTML] Primer design for quantitative real-time PCR for the emerging Coronavirus SARS-CoV-2

D Li, J Zhang, J Li - Theranostics, 2020 - ncbi.nlm.nih.gov
In December 2019, a new coronavirus disease (COVID-19) outbreak occurred in Wuhan,
China. Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), which is the …

[PDF][PDF] A critical review on PCR, its types and applications

J Singh, N Birbian, S Sinha, A Goswami - Int. J. Adv. Res. Biol. Sci, 2014 - researchgate.net
The invention of polymerase chain reaction (PCR) has been a milestone in the history of
biological and medical sciences. The applications of PCR have not only completely …

Design of primers and probes for quantitative real-time PCR methods

A Rodríguez, M Rodríguez, JJ Córdoba, MJ Andrade - PCR primer design, 2015 - Springer
Abstract Design of primers and probes is one of the most crucial factors affecting the success
and quality of quantitative real-time PCR (qPCR) analyses, since an accurate and reliable …

LettuceGDB: the community database for lettuce genetics and omics

Z Guo, B Li, J Du, F Shen, Y Zhao, Y Deng, Z Kuang… - Plant …, 2023 - cell.com
As a globally popular leafy vegetable and a representative plant of the Asteraceae family,
lettuce has great economic and academic significance. In the last decade, high-throughput …

The influence of quality of primers on the formation of primer dimers in PCR

RR Garafutdinov, AA Galimova… - … Nucleotides & Nucleic …, 2020 - Taylor & Francis
Polymerase chain reaction (PCR) is the most commonly used method for nucleic acids
amplification. PCR performance depends on several causes, among which the quality of …

Differential expression of an endogenous retroviral element [HERV-K (HML-6)] is associated with reduced survival in glioblastoma patients

AH Shah, V Govindarajan, TT Doucet-O'Hare… - Scientific Reports, 2022 - nature.com
Comprising approximately 8% of our genome, Human Endogenous RetroViruses (HERVs)
represent a class of germline retroviral infections that are regulated through epigenetic …

[HTML][HTML] Discovery of novel peptide neurotoxins from sea anemone species

J Fu, Y Liao, AH Jin, B Gao - Frontiers in Bioscience-Landmark, 2021 - imrpress.com
As primitive metazoa, sea anemones are rich in various bioactive peptide neurotoxins.
These peptides have been applied to neuroscience research tools or directly developed as …

Simple, quick and cost-efficient: A universal RT-PCR and sequencing strategy for genomic characterisation of foot-and-mouth disease viruses

V Dill, M Beer, B Hoffmann - Journal of virological methods, 2017 - Elsevier
Foot-and-mouth disease (FMD) is a major contributor to poverty and food insecurity in Africa
and Asia, and it is one of the biggest threats to agriculture in highly developed countries. As …

[HTML][HTML] Plasmonic materials and manufacturing methods for rapid and sustainable thermal cycler for PCR

K Shrestha, S Kim, G Cho - Materials Today Advances, 2023 - Elsevier
Multiple outbreaks of fatal infectious diseases throughout history have intensified the need
for early diagnostic methods to efficiently control their spread. Polymerase chain reaction …

Разнообразие праймеров для ПЦР и принципы их подбора

РР Гарафутдинов, АХ Баймиев, ГВ Малеев… - Биомика, 2019 - elibrary.ru
Праймеры являются очень важным компонентом ПЦР, поскольку специфичность
амплификации зависит в первую очередь от них. Хотя на эффективность ПЦР влияет …