New developments in RiPP discovery, enzymology and engineering
M Montalbán-López, TA Scott, S Ramesh… - Natural product …, 2021 - pubs.rsc.org
Covering: up to June 2020 Ribosomally-synthesized and post-translationally modified
peptides (RiPPs) are a large group of natural products. A community-driven review in 2013 …
peptides (RiPPs) are a large group of natural products. A community-driven review in 2013 …
Macrocyclization and backbone modification in RiPP biosynthesis
H Lee, WA Van Der Donk - Annual review of biochemistry, 2022 - annualreviews.org
The past decade has seen impressive advances in understanding the biosynthesis of
ribosomally synthesized and posttranslationally modified peptides (RiPPs). One of the most …
ribosomally synthesized and posttranslationally modified peptides (RiPPs). One of the most …
Substrate-controlled catalysis in the ether cross-link-forming radical SAM enzymes
Darobactin is a heptapeptide antibiotic featuring an ether cross-link and a C–C cross-link,
and both cross-links are installed by a radical S-adenosylmethionine (rSAM) enzyme DarE …
and both cross-links are installed by a radical S-adenosylmethionine (rSAM) enzyme DarE …
Emulating nonribosomal peptides with ribosomal biosynthetic strategies
S Mordhorst, F Ruijne, AL Vagstad, OP Kuipers… - RSC Chemical …, 2023 - pubs.rsc.org
Peptide natural products are important lead structures for human drugs and many
nonribosomal peptides possess antibiotic activity. This makes them interesting targets for …
nonribosomal peptides possess antibiotic activity. This makes them interesting targets for …
Selection for constrained peptides that bind to a single target protein
Peptide secondary metabolites are common in nature and have diverse pharmacologically-
relevant functions, from antibiotics to cross-kingdom signaling. Here, we present a method to …
relevant functions, from antibiotics to cross-kingdom signaling. Here, we present a method to …
Current advances in design and engineering strategies of industrial enzymes
T Dinmukhamed, Z Huang, Y Liu, X Lv, J Li… - Systems Microbiology …, 2021 - Springer
Enzymes are considered as functional proteins, also known as biocatalysts, which are
required for normal cellular function. The commercial production of enzymes with distinct …
required for normal cellular function. The commercial production of enzymes with distinct …
Post‐Translational Formation of Aminomalonate by a Promiscuous Peptide‐Modifying Radical SAM Enzyme
Aminomalonate (Ama) is a widespread structural motif in Nature, whereas its biosynthetic
route is only partially understood. In this study, we show that a radical S …
route is only partially understood. In this study, we show that a radical S …
Leveraging Substrate Promiscuity of a Radical S-Adenosyl-L-methionine RiPP Maturase toward Intramolecular Peptide Cross-Linking Applications
KAS Eastman, WM Kincannon… - ACS Central Science, 2022 - ACS Publications
Radical S-adenosyl-l-methionine (RS) enzymes operate on a variety of substrates and
catalyze a wide range of complex radical-mediated transformations. Radical non-α-carbon …
catalyze a wide range of complex radical-mediated transformations. Radical non-α-carbon …
Cysteinyl radicals in chemical synthesis and in nature
Nature harnesses the unique properties of cysteinyl radical intermediates for a diverse
range of essential biological transformations including DNA biosynthesis and repair …
range of essential biological transformations including DNA biosynthesis and repair …
How a subfamily of radical S-adenosylmethionine enzymes became a mainstay of ribosomally synthesized and post-translationally modified peptide discovery
Radical S-adenosylmethionine (rSAM) enzymes are a large and diverse superfamily of
enzymes, some of which are known to participate in the biosynthesis of ribosomally …
enzymes, some of which are known to participate in the biosynthesis of ribosomally …