Lab-to-field transition of RNA spray applications–how far are we?

AP Rank, A Koch - Frontiers in Plant Science, 2021 - frontiersin.org
The drastic loss of biodiversity has alarmed the public and raised sociopolitical demand for
chemical pesticide-free plant production, which is now treated by governments worldwide as …

RNAi-based pest control: Production, application and the fate of dsRNA

L He, Y Huang, X Tang - Frontiers in Bioengineering and …, 2022 - frontiersin.org
The limitations of conventional pesticides have raised the demand for innovative and
sustainable solutions for plant protection. RNA Interference (RNAi) triggered by dsRNA has …

Off-target effects of RNAi correlate with the mismatch rate between dsRNA and non-target mRNA

J Chen, Y Peng, H Zhang, K Wang, C Zhao, G Zhu… - RNA biology, 2021 - Taylor & Francis
RNAi is a potent technique for the knockdown of target genes. However, its potential off-
target effects limit the widespread applications in both reverse genetic analysis and genetic …

Next generation dsRNA-based insect control: Success so far and challenges

RB Nitnavare, J Bhattacharya, S Singh… - Frontiers in Plant …, 2021 - frontiersin.org
RNA interference (RNAi) is a method of gene silencing where dsRNA is digested into small
interfering RNA (siRNA) in the presence of enzymes. These siRNAs then target homologous …

Recent advances in understanding of the mechanisms of RNA interference in insects

J Koo, SR Palli - Insect Molecular Biology, 2024 - Wiley Online Library
We highlight the recent 5 years of research that contributed to our understanding of the
mechanisms of RNA interference (RNAi) in insects. Since its first discovery, RNAi has …

RNAi targets in agricultural pest insects: advancements, knowledge gaps, and IPM

J Willow, CNT Taning, SM Cook, S Sulg, AI Silva… - Frontiers in …, 2021 - frontiersin.org
The unprecedented target-specificity of double-stranded RNA (dsRNA), due to its sequence-
specific mode of action, puts dsRNA at the forefront of biosafe insecticide technology. Since …

Identification of a double-stranded RNA-degrading nuclease influencing both ingestion and injection RNA interference efficiency in the red flour beetle Tribolium …

Y Peng, K Wang, J Chen, J Wang, H Zhang, L Ze… - Insect biochemistry and …, 2020 - Elsevier
RNA interference (RNAi) efficiency dramatically varies among different insects and among
administration methods. Numerous studies have revealed that a poor RNAi response is …

Knockdown of double-stranded RNases (dsRNases) enhances oral RNA interference (RNAi) in the corn leafhopper, Dalbulus maidis

LI Dalaisón-Fuentes, A Pascual, M Crespo… - Pesticide Biochemistry …, 2023 - Elsevier
The leafhopper Dalbulus maidis is a harmful pest that causes severe damage to corn crops.
Conventional chemical pesticides have negative environmental impacts, emphasizing the …

A dsRNA‐degrading nuclease (dsRNase2) limits RNAi efficiency in the Asian corn borer (Ostrinia furnacalis)

YH Fan, HF Song, M Abbas, YL Wang, T Li… - Insect …, 2021 - Wiley Online Library
The efficiency of RNA interference (RNAi) varies substantially among different insect
species. Rapid degradation of double‐stranded RNA (dsRNA) by dsRNA‐degrading …

Double-stranded RNA degrading nuclease affects RNAi efficiency in the melon fly, Zeugodacus cucurbitae

S Ahmad, M Jamil, CC Jaworski, Y Luo - Journal of Pest Science, 2024 - Springer
RNA interference (RNAi) is an efficient, species-specific technology to control invasive pests.
However, its efficacy varies among insect species, notably due to the presence of double …