From its start as a small‐scale in vitro system to study fundamental translation processes,
cell‐free protein synthesis quickly rose to become a potent platform for the high‐yield …

Incorporating non-standard amino acids (NSAAs) into proteins enables new chemical
properties, new structures, and new functions. In recent years, improvements in cell-free …

Biotherapeutics have many promising applications, such as anti‐cancer treatments, immune
suppression, and vaccines. However, due to their biological nature, some biotherapeutics …

Synthetic biology transforms the way we perceive biological systems. Emerging
technologies in this field affect many disciplines of science and engineering. Traditionally …

Cell‐free protein synthesis (CFPS) systems allow for robust protein expression with easy
manipulation of conditions to improve protein yield and folding. Recent technological …

Highlights•PCR products can be used to make proteins quickly and easily in a cell-free
system.•Recent advances have widened the application space of such cell-free …

Cell-free protein synthesis (CFPS) is a versatile tool for rapid recombinant protein production
and engineering. One drawback of cell-free technology is the necessity to store the major …

Many diseases and disorders are linked to exposure to endocrine disrupting chemicals
(EDCs) that mimic the function of natural estrogen hormones. Here we present a Rapid …

Approximately one third of protein therapeutics are produced in Escherichia coli, targeting a
wide variety of diseases. However, due to immune recognition of endotoxin (a lipid …

Protein production using processed cell lysates is a core technology in synthetic biology and
these systems are excellent to produce difficult toxins or membrane proteins. However, the …