Crosslink cleaving enzymes: the smart autolysins that remodel the bacterial cell wall

V Rajguru, S Chatterjee, S Garde, M Reddy - Trends in Microbiology, 2024 - cell.com
Peptidoglycan (PG) is a protective mesh-like polymer in bacterial cell walls that enables their
survival in almost every ecological niche. PG is formed by crosslinking of several glycan …

Mechanistic insights into the activities of major families of enzymes in bacterial peptidoglycan assembly and breakdown

JMC Kwan, Y Qiao - ChemBioChem, 2023 - Wiley Online Library
Serving as an exoskeletal scaffold, peptidoglycan is a polymeric macromolecule that is
essential and conserved across all bacteria, yet is absent in mammalian cells; this has made …

Bioinformatic characterization of endolysins and holin-like membrane proteins in the lysis cassette of phages that infect Gordonia rubripertincta

RS Pollenz, J Bland, WH Pope - PLoS One, 2022 - journals.plos.org
Holins are bacteriophage-encoded transmembrane proteins that function to control the
timing of bacterial lysis event, assist with the destabilization of the membrane proton motive …

Asymmetric peptidoglycan editing generates cell curvature in Bdellovibrio predatory bacteria

EJ Banks, M Valdivia-Delgado, J Biboy… - Nature …, 2022 - nature.com
Peptidoglycan hydrolases contribute to the generation of helical cell shape in
Campylobacter and Helicobacter bacteria, while cytoskeletal or periskeletal proteins …

The Role of luxS in Campylobacter jejuni Beyond Intercellular Signaling

D Ramić, B Jug, K Šimunović… - Microbiology …, 2023 - Am Soc Microbiol
The full role of the luxS gene in the biological processes, such as essential amino acid
synthesis, nitrogen and pyruvate metabolism, and flagellar assembly, of Campylobacter …

Multiple Campylobacter jejuni proteins affecting the peptidoglycan structure and the degree of helical cell curvature

E Frirdich, J Vermeulen, J Biboy, W Vollmer… - Frontiers in …, 2023 - frontiersin.org
Campylobacter jejuni is a Gram-negative helical bacterium. Its helical morphology,
maintained by the peptidoglycan (PG) layer, plays a key role in its transmission in the …

Characterization of VldE (Spr1875), a Pneumococcal Two-State l,d-Endopeptidase with a Four-Zinc Cluster in the Active Site

V Miguel-Ruano, I Acebrón, M Lee… - ACS …, 2024 - ACS Publications
Remodeling of the pneumococcal cell wall, carried out by peptidoglycan (PG) hydrolases, is
imperative for maintaining bacterial cell shape and ensuring survival, particularly during cell …

The choice of chromatographic resin for the purification of recombinant lysostaphin affects its activity

NV Shestak, AV Grishin, AM Lyashchuk… - Protein expression and …, 2023 - Elsevier
Lysostaphin is a zinc-dependent endopeptidase that is effective against both antibiotic-
sensitive and antibiotic-resistant strains of Staphylococcus aureus. Lysostaphin is typically …

Purification and characterizations of a novel extracellular protease from Shewanella putrefaciens isolated from bigeye tuna

Z Yi, J Yan, Z Ding, J Xie - Food Bioscience, 2023 - Elsevier
Shewanella putrefaciens is one of the common spoilage microorganisms in aquatic
products, and its extracellular protease is the main cause of seafood spoilage. The …

One fold, many functions—M23 family of peptidoglycan hydrolases

A Razew, JN Schwarz, P Mitkowski, I Sabala… - Frontiers in …, 2022 - frontiersin.org
Bacterial cell walls are the guards of cell integrity. They are composed of peptidoglycan that
provides rigidity to sustain internal turgor and ensures isolation from the external …