The cre‐loxP–mediated recombination system (the “cre‐loxP system”) is an integral
experimental tool for mammalian genetics and cell biology. Use of the system has greatly …

Mouse models of human cancer have played a vital role in understanding tumorigenesis
and answering experimental questions that other systems cannot address. Advances …

Unicellular organisms such as yeasts require a single cyclin-dependent kinase, Cdk1, to
drive cell division. In contrast, mammalian cells are thought to require the sequential …

Tyrosine site-specific recombinases (SSRs) including Cre and FLP are essential tools for
DNA and genome engineering. Cre has long been recognized as the best SSR for genome …

Determining the mechanism of gene function is greatly enhanced using conditional
mutagenesis. However, generating engineered conditional alleles is inefficient and has only …

The microRNA-183/96/182 cluster is highly expressed in the retina and other sensory
organs. To uncover its in vivo functions in the retina, we generated a knockout mouse model …

Cohesin is a protein complex originally identified for its role in sister chromatid cohesion,
although increasing evidence portrays it also as a major organizer of interphase chromatin …

Genetic engineering is the use of molecular biology technology to modify DNA sequence (s)
in genomes, using a variety of approaches. For example, homologous recombination can be …

Tubulin glutamylation is a post-translational modification that occurs predominantly in the
ciliary axoneme and has been suggested to be important for ciliary function,. However, its …

NMNAT2 is an NAD+-synthesizing enzyme with an essential axon maintenance role in
primary culture neurons. We have generated an Nmnat2 gene trap mouse to examine the …