The comet assay for DNA damage and repair: principles, applications, and limitations

AR Collins - Molecular biotechnology, 2004 - Springer
The comet assay (single-cell gel electrophoresis) is a simple method for measuring
deoxyribonucleic acid (DNA) strand breaks in eukaryotic cells. Cells embedded in agarose …

The comet assay: a comprehensive review

DW Fairbairn, PL Olive, KL O'Neill - Mutation Research/Reviews in Genetic …, 1995 - Elsevier
The comet assay is a sensitive and rapid method for DNA strand break detection in
individual cells. Its use has increased significantly in the past few years. This paper is a …

Use of the single cell gel electrophoresis/comet assay for detecting DNA damage in aquatic (marine and freshwater) animals

RF Lee, S Steinert - Mutation Research/Reviews in Mutation Research, 2003 - Elsevier
The comet assay is a rapid, sensitive and inexpensive method for measuring DNA strand
breaks. The comet assay has advantages over other DNA damage methods, such as sister …

The single cell gel electrophoresis assay (comet assay): a European review

VJ McKelvey-Martin, MHL Green, P Schmezer… - Mutation Research …, 1993 - Elsevier
The single cell gel electrophoresis (SCGE) assay is a rapid, simple, visual and sensitive
technique for measuring DNA breakage in individual mammalian cells. Here we review the …

Single cell gel electrophoresis assay: methodology and applications

E Rojas, MC Lopez, M Valverde - Journal of Chromatography B …, 1999 - Elsevier
The single cell gel electrophoresis or Comet assay is a sensitive, reliable, and rapid method
for DNA double-and single-strand breaks, alkali-labile sites and delayed repair site …

Effects of flavonoids and vitamin C on oxidative DNA damage to human lymphocytes

M Noroozi, WJ Angerson, ME Lean - The American journal of clinical …, 1998 - Elsevier
This study assessed the antioxidant potencies of several widespread dietary flavonoids
across a range of concentrations and compared with vitamin C as a positive control. The …

The comet assay (Single-Cell Gel Test) a sensitive genotoxicity test for the detection of DNA damage and repair

G Speit, A Hartmann - DNA repair protocols: eukaryotic systems, 1999 - Springer
The comet assay or single-cell gel (SCG) test is becoming established as a useful technique
for studying DNA damage and repair. In this microgel electrophoresis technique, a small …

Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells

CM Gedik, SWB Ewen, AR Collins - International journal of …, 1992 - Taylor & Francis
DNA breaks in eukaryotic cells can be detected by alkaline electrophoresis of cells
embedded in agarose. DNA containing breaks extends in the direction of the anode forming …

Effects of lycopene supplementation on oxidative stress and markers of endothelial function in healthy men

JY Kim, JK Paik, OY Kim, HW Park, JH Lee, Y Jang… - Atherosclerosis, 2011 - Elsevier
OBJECTIVE: The objective was to determine the effects of lycopene supplementation on
endothelial function assessed by reactive hyperemia peripheral arterial tonometry (RH-PAT) …

The comet assay: a sensitive genotoxicity test for the detection of DNA damage and repair

G Speit, A Hartmann - DNA Repair Protocols: Mammalian Systems, 2006 - Springer
The comet assay (single-cell gel electrophoresis) is a simple and sensitive method for
studying DNA damage and repair. In this microgel electrophoresis technique, a small …