The ever-expanding set of CRISPR technologies and their programmable RNA-guided
nucleases exhibit remarkable flexibility in DNA targeting. However, this flexibility comes with …

Background Delivery of CRISPR reagents into cells as ribonucleoprotein (RNP) complexes
enables transient editing, and avoids CRISPR reagent integration in the genomes. Another …

Targeting definite genomic locations using CRISPR-Cas systems requires a set of enzymes
with unique protospacer adjacent motif (PAM) compatibilities. To expand this repertoire, we …

CRISPR-Cas systems comprise diverse adaptive immune systems in prokaryotes whose
RNA-directed nucleases have been co-opted for various technologies. Recent efforts have …

CRISPR technologies have overwhelmingly relied on the Streptococcus pyogenes Cas9
(SpyCas9), with its consensus NGG and less preferred NAG and NGA protospacer-adjacent …

CRISPR-associated (Cas) nucleases are multifunctional tools for gene editing. Cas12a
possesses several advantages, including the requirement of a single guide RNA and high …

CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR
associated protein) genome editing is a powerful technology widely used in current genetic …

CRISPR-Cas systems have proven effective in a variety of applications due to their ease of
use and relatively high editing efficiency. Yet, any individual CRISPR-Cas system has …

Type V CRISPR-Cas effectors have revolutionized molecular diagnostics by facilitating the
detection of nucleic acid biomarkers. However, their dependence on the presence of …

Rice (Oryza sativa L.) no doubt is an important staple food crop which feeds more than 3
billion people and provides 20% of world's dietary energy (Mishra et al., 2018). Its global …