Advances and challenges in metatranscriptomic analysis

M Shakya, CC Lo, PSG Chain - Frontiers in genetics, 2019 - frontiersin.org
Sequencing-based analyses of microbiomes have traditionally focused on addressing the
question of community membership and profiling taxonomic abundance through amplicon …

[PDF][PDF] A simple guide to de novo transcriptome assembly and annotation

V Raghavan, L Kraft, F Mesny… - Briefings in …, 2022 - academic.oup.com
A transcriptome constructed from short-read RNA sequencing (RNA-seq) is an easily
attainable proxy catalog of protein-coding genes when genome assembly is unnecessary …

Using SPAdes de novo assembler

A Prjibelski, D Antipov, D Meleshko… - Current protocols in …, 2020 - Wiley Online Library
Abstract SPAdes—St. Petersburg genome Assembler—was originally developed for de
novo assembly of genome sequencing data produced for cultivated microbial isolates and …

Wildfire-dependent changes in soil microbiome diversity and function

AR Nelson, AB Narrowe, CC Rhoades, TS Fegel… - Nature …, 2022 - nature.com
Forest soil microbiomes have crucial roles in carbon storage, biogeochemical cycling and
rhizosphere processes. Wildfire season length, and the frequency and size of severe fires …

Coral microbiome manipulation elicits metabolic and genetic restructuring to mitigate heat stress and evade mortality

EP Santoro, RM Borges, JL Espinoza, M Freire… - Science …, 2021 - science.org
Beneficial microorganisms for corals (BMCs) ameliorate environmental stress, but whether
they can prevent mortality and the underlying host response mechanisms remains elusive …

Petabase-scale sequence alignment catalyses viral discovery

RC Edgar, B Taylor, V Lin, T Altman, P Barbera… - Nature, 2022 - nature.com
Public databases contain a planetary collection of nucleic acid sequences, but their
systematic exploration has been inhibited by a lack of efficient methods for searching this …

De novo transcriptome assembly: A comprehensive cross-species comparison of short-read RNA-Seq assemblers

M Hölzer, M Marz - Gigascience, 2019 - academic.oup.com
Background In recent years, massively parallel complementary DNA sequencing (RNA
sequencing [RNA-Seq]) has emerged as a fast, cost-effective, and robust technology to …

Genomic impact of stress-induced transposable element mobility in Arabidopsis

D Roquis, M Robertson, L Yu, M Thieme… - Nucleic acids …, 2021 - academic.oup.com
Transposable elements (TEs) have long been known to be major contributors to plant
evolution, adaptation and crop domestication. Stress-induced TE mobilization is of particular …

Metagenomic data assembly–the way of decoding unknown microorganisms

AL Lapidus, AI Korobeynikov - Frontiers in Microbiology, 2021 - frontiersin.org
Metagenomics is a segment of conventional microbial genomics dedicated to the
sequencing and analysis of combined genomic DNA of entire environmental samples. The …

[PDF][PDF] De novo assembly of 20 chicken genomes reveals the undetectable phenomenon for thousands of core genes on microchromosomes and subtelomeric regions

M Li, C Sun, N Xu, P Bian, X Tian… - Molecular biology …, 2022 - academic.oup.com
The gene numbers and evolutionary rates of birds were assumed to be much lower than
those of mammals, which is in sharp contrast to the huge species number and …