Emerging models of the bacterial nucleoid show that nucleoid-associated proteins (NAPs)
and transcription contribute in combination to the dynamic nature of nucleoid structure …

If fully stretched out, a typical bacterial chromosome would be nearly 1 mm long,
approximately 1,000 times the length of a cell. Not only must cells massively compact their …

Chromosomes must be highly compacted and organized within cells, but how this is
achieved in vivo remains poorly understood. We report the use of chromosome conformation …

Transcription generates local topological and mechanical constraints on the DNA fiber,
leading to the generation of supercoiled chromosome domains in bacteria. However, the …

Transcription of highly expressed genes has been shown to occur in stochastic bursts. But
the origin of such ubiquitous phenomenon has not been understood. Here, we present the …

In eukaryotes, the location of a gene on the chromosome is known to affect its expression,
but such position effects are poorly understood in bacteria. Here, using Escherichia coli K …

Chromosomes of a broad range of species, from bacteria to mammals, are structured by
large topological domains whose precise functional roles and regulatory mechanisms …

The presence and biological importance of DNA secondary structures in eukaryotic
promoters are becoming increasingly recognized among chemists and biologists as …

How genomes are organized within cells and how the 3D architecture of a genome
influences cellular functions are significant questions in biology. A bacterial genomic DNA …

The bacterial chromosome must be compacted more than 1,000-fold to fit into the
compartment in which it resides. How it is condensed, organized and ultimately segregated …