The eukaryotic 26S proteasome is a large multisubunit complex that degrades the majority
of proteins in the cell under normal conditions. The 26S proteasome can be divided into two …

The proteasome has emerged as an intricate machine that has dynamic mechanisms to
regulate the timing of its activity, its selection of substrates, and its processivity. The 19 …

We report the cryo-EM structure of the human 26S proteasome at an average resolution of
3.5 Å, allowing atomic modeling of 28 subunits in the core particle (CP) and 18 subunits in …

The ubiquitin proteasome system (UPS) is the main ATP-dependent protein degradation
pathway in the cytosol and nucleus of eukaryotic cells. At its centre is the 26S proteasome …

Protein degradation in eukaryotic cells is performed by the Ubiquitin-Proteasome System
(UPS). The 26S proteasome holocomplex consists of a core particle (CP) that proteolytically …

The 26S proteasome is a 2.5 MDa molecular machine that executes the degradation of
substrates of the ubiquitin–proteasome pathway. The molecular architecture of the 26S …

Abstract Ubiquitin Proteasome System (UPS) is an adaptable and finely tuned system that
sustains proteostasis network under a large variety of physiopathological conditions. Its …

We describe a method that integrates data derived from different mass spectrometry (MS)-
based techniques with a modeling strategy for structural characterization of protein …

The 26S proteasome is responsible for the selective, ATP-dependent degradation of
polyubiquitinated cellular proteins. Removal of ubiquitin chains from targeted substrates at …

Many full-length proteins and protein regions lack stable tertiary and/or secondary structure
under physiological conditions in vitro. These proteins and regions, known as intrinsically …