Efficacy validation of SARS-CoV-2-inactivation and viral genome stability in saliva by a guanidine hydrochloride and surfactant-based virus lysis/transport buffer

JG Komu, D Jamsransuren, S Matsuda, H Ogawa… - Viruses, 2023 - mdpi.com
To enhance biosafety and reliability in SARS-CoV-2 molecular diagnosis, virus
lysis/transport buffers should inactivate the virus and preserve viral RNA under various …

Collection and transportation of SARS-CoV-2 and influenza A virus diagnostic samples: Optimizing the usage of guanidine-based chaotropic salts for enhanced …

JG Komu, D Jamsransuren, S Matsuda… - Biochemical and …, 2024 - Elsevier
Many virus lysis/transport buffers used in molecular diagnostics, including the detection of
severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA, contain guanidine …

[HTML][HTML] Optimization of nucleic acid extraction methods for rapid detection in pandemic situations or diseases with high prevalence

HL Wiraswati, IF Ma'ruf, S Ekawardhani… - Journal of …, 2023 - ncbi.nlm.nih.gov
Nucleic acid extraction (NAE) is an essential component of many assays used for diagnostic
and research purposes. During a pandemic, performing NAE is associated with significant …

Diagnostic accuracy of direct reverse transcription-polymerase chain reaction using guanidine-based and guanidine-free inactivators for SARS-CoV-2 detection in …

T Katsuno, M Kimura, J Terada-Hirashima… - Journal of Virological …, 2024 - Elsevier
This study aimed to evaluate diagnostic accuracy of SARS-CoV-2 RNA detection in saliva
samples treated with a guanidine-based or guanidine-free inactivator, using …

[HTML][HTML] The Establishment of the Spiking Method to Evaluate the Rapid Diagnostic Test Antigen (Ag-RDT) Product for COVID-19 Detection

HL Wiraswati, L Faridah… - Biomedical and …, 2024 - biomedpharmajournal.org
The quality control of the COVID-19 Rapid Diagnostic Test (Ag-RDT) product is regarded as
one of the government's responsibilities. The Indonesian government establishes rules for …

[HTML][HTML] Effects of storage temperature on throat swabs preserved in different transport media for detection of SARS-CoV-2 by reverse transcriptase polymerase chain …

L Verma, S Hooja, R Yadav, N Pal, K Ranawat… - Journal of Laboratory … - jlabphy.org
Objectives: The coronavirus disease 2019 (COVID-19) pandemic resulted in an increased
need for molecular diagnostic testing. Delay in the specimen processing and storage of …