Current progress in computational structure-based protein design is reviewed in the areas of
methodology and applications. Foundational advances include new potential functions …

Data‐driven protein engineering is increasingly used as an alternative to rational design
and combinatorial engineering because it uses available knowledge to limit library size …

A long-standing problem in molecular biology is the determination of a complete functional
conformational landscape of proteins. This includes not only proteins' native structures, but …

The newly emerging field of systems biology involves a judicious interplay between high‐
throughput 'wet'experimentation, computational modelling and technology development …

Background Intrabodies are defined as antibody molecules which are ectopically expressed
inside the cell. Such intrabodies can be used to visualize or inhibit the targeted antigen in …

Computational protein design continues to experience a variety of methodological
advances. Several improvements have been suggested for the objective functions used to …

Engineering proteins that can fold to unique structures remains a challenge. Protein stability
has previously been engineered via the observed correlation between thermal stability and …

Ferritin four-helix bundle subunits self-assemble to create a stable multimer with a large
central hydrophilic cavity where metal ions bind. To explore the versatility of this reaction …

Computational and theoretical methods are advancing protein design as a means to create
and investigate proteins. Such efforts further our capacity to control, design and understand …

Membrane proteins are involved in a wide variety of cellular processes, and are typically
part of the first interaction a cell has with extracellular molecules. As a result, these proteins …