[HTML][HTML] Agrobacterium-mediated plant transformation: biology and applications

HH Hwang, M Yu, EM Lai - The arabidopsis book, 2017 - ncbi.nlm.nih.gov
Plant genetic transformation heavily relies on the bacterial pathogen Agrobacterium
tumefaciens as a powerful tool to deliver genes of interest into a host plant. Inside the plant …

VIGS, HIGS and FIGS: small RNA silencing in the interactions of viruses or filamentous organisms with their plant hosts

DC Baulcombe - Current opinion in plant biology, 2015 - Elsevier
Highlights•RNA produced in a host plant can affect gene expression in an interacting fungus
or oomycete.•RNA produced in a fungus can affect the defense system of a host …

Genetically modified organism-free RNA interference: exogenous application of RNA molecules in plants

A Dalakouras, M Wassenegger, E Dadami… - Plant …, 2020 - academic.oup.com
Genetically Modified Organism-Free RNA Interference: Exogenous Application of RNA Molecules
in Plants1 [OPEN] | Plant Physiology | Oxford Academic Skip to Main Content Advertisement …

Respective contributions of Arabidopsis DCL 2 and DCL 4 to RNA silencing

JS Parent, N Bouteiller, T Elmayan… - The Plant …, 2015 - Wiley Online Library
Dicer proteins are central to the different mechanisms involving RNA interference. Plants
have evolved multiple DICER‐LIKE (DCL) copies, thus enabling functional diversification. In …

Induction of Silencing in Plants by High-Pressure Spraying of In vitro-Synthesized Small RNAs

A Dalakouras, M Wassenegger, JN McMillan… - Frontiers in plant …, 2016 - frontiersin.org
In this report, we describe a method for the delivery of small interfering RNAs (siRNAs) into
plant cells. In vitro synthesized siRNAs that were designed to target the coding region of a …

Artificial microRNA mediated gene silencing in plants: progress and perspectives

M Tiwari, D Sharma, PK Trivedi - Plant molecular biology, 2014 - Springer
Homology based gene silencing has emerged as a convenient approach for repressing
expression of genes in order to study their functions. For this purpose, several antisense or …

The key role of terminators on the expression and post‐transcriptional gene silencing of transgenes

FF de Felippes, M McHale, RL Doran… - The Plant …, 2020 - Wiley Online Library
Transgenes have become essential to modern biology, being an important tool in functional
genomic studies and also in the development of biotechnological products. One of the major …

Barriers to efficient foliar uptake of dsRNA and molecular barriers to dsRNA activity in plant cells

M Bennett, J Deikman, B Hendrix… - Frontiers in plant …, 2020 - frontiersin.org
Foliar application of dsRNA to elicit an RNA interference (RNAi) response is currently under
consideration as a crop protection strategy. To access the RNAi machinery of a plant, foliarly …

Arabidopsis leaf‐expressed AGAMOUS‐LIKE 24 mRNA systemically specifies floral meristem differentiation

NC Huang, HC Tien, TS Yu - New Phytologist, 2024 - Wiley Online Library
Plants can record external stimuli in mobile mRNAs and systemically deliver them to distal
tissues to adjust development. Despite the identification of thousands of mobile mRNAs, the …

Plin‐amiR, a pre‐microRNA‐based technology for controlling herbivorous insect pests

J Bally, E Fishilevich, RL Doran, K Lee… - Plant Biotechnology …, 2020 - Wiley Online Library
The cotton bollworm, Helicoverpa armigera, is a major insect pest for a wide range of
agricultural crops. It causes significant yield loss through feeding damage and by increasing …