Water in nanopores and biological channels: a molecular simulation perspective

CI Lynch, S Rao, MSP Sansom - Chemical reviews, 2020 - ACS Publications
This Review explores the dynamic behavior of water within nanopores and biological
channels in lipid bilayer membranes. We focus on molecular simulation studies, alongside …

Electroosmosis in nanopores: computational methods and technological applications

A Gubbiotti, M Baldelli, G Di Muccio… - … in Physics: X, 2022 - Taylor & Francis
Electroosmosis is a fascinating effect where liquid motion is induced by an applied electric
field. Counter ions accumulate in the vicinity of charged surfaces, triggering a coupling …

Translocation of linearized full-length proteins through an engineered nanopore under opposing electrophoretic force

A Sauciuc, B Morozzo della Rocca, MJ Tadema… - Nature …, 2023 - nature.com
Nanopores have recently been used to identify and fingerprint proteins. However, because
proteins, unlike DNA, do not have a uniform charge, the electrophoretic force cannot in …

Protein Sizing with 15 nm Conical Biological Nanopore YaxAB

S Straathof, G Di Muccio, M Yelleswarapu… - ACS …, 2023 - ACS Publications
Nanopores are promising single-molecule tools for the electrical identification and
sequencing of biomolecules. However, the characterization of proteins, especially in real …

Controlled movement of ssDNA conjugated peptide through Mycobacterium smegmatis porin A (MspA) nanopore by a helicase motor for peptide sequencing …

Z Chen, Z Wang, Y Xu, X Zhang, B Tian, J Bai - Chemical science, 2021 - pubs.rsc.org
The lack of an efficient, low-cost sequencing method has long been a significant bottleneck
in protein research and applications. In recent years, the nanopore platform has emerged as …

Analytical model for particle capture in nanopores elucidates competition among electrophoresis, electroosmosis, and dielectrophoresis

M Chinappi, M Yamaji, R Kawano, F Cecconi - ACS nano, 2020 - ACS Publications
The interaction between nanoparticles dispersed in a fluid and nanopores is governed by
the interplay of hydrodynamical, electrical, and chemical effects. We developed a theory for …

β-Barrel nanopores with an acidic–aromatic sensing region identify proteinogenic peptides at low pH

RCA Versloot, SAP Straathof, G Stouwie, MJ Tadema… - ACS …, 2022 - ACS Publications
Biological nanopores are emerging as sensitive single-molecule sensors for proteins and
peptides. The heterogeneous charge of a polypeptide chain, however, can complicate or …

The manipulation of the internal hydrophobicity of FraC nanopores augments peptide capture and recognition

FLR Lucas, K Sarthak, EM Lenting, D Coltan… - ACS …, 2021 - ACS Publications
The detection of analytes and the sequencing of DNA using biological nanopores have seen
major advances over recent years. The analysis of proteins and peptides with nanopores …

Nanopore‐based protein sequencing using biopores: Current achievements and open challenges

A Asandei, G Di Muccio, I Schiopu, L Mereuta… - Small …, 2020 - Wiley Online Library
The synergy of life sciences discoveries, biomolecular and protein engineering advances,
and groundbreaking nanofabrication technologies, has introduced over the past years the …

Protein sequencing via nanopore based devices: a nanofluidics perspective

M Chinappi, F Cecconi - Journal of Physics: Condensed Matter, 2018 - iopscience.iop.org
Proteins perform a huge number of central functions in living organisms, thus all the new
techniques allowing their precise, fast and accurate characterization at single-molecule level …