The centromere performs a universally conserved function, to accurately partition genetic
information upon cell division. Yet, centromeres are among the most rapidly evolving …

Chromatin compacts genomic DNA in eukaryotes. The primary chromatin unit is the
nucleosome core particle, composed of four pairs of the core histones, H2A, H2B, H3, and …

Structural heterogeneity of nucleosomes in functional chromosomes is unknown. Here, we
devise the template-, reference-and selection-free (TRSF) cryo-EM pipeline to …

The histone H3 variant CENP-A is a crucial epigenetic marker for centromere specification.
CENP-A forms a characteristic nucleosome and dictates the higher-order configuration of …

Centromeric nucleosomes are at the interface of the chromosome and the kinetochore that
connects to spindle microtubules in mitosis. The core centromeric nucleosome complex …

Subcellular compartmentalization of metabolic enzymes establishes a unique metabolic
environment that elicits specific cellular functions. Indeed, the nuclear translocation of …

Centromeres are defined epigenetically by nucleosomes containing the histone H3 variant
CENP‐A, upon which the constitutive centromere‐associated network of proteins (CCAN) is …

The CENP‐A nucleosome is a key structure for kinetochore assembly. Once the CENP‐A
nucleosome is established in the centromere, additional proteins recognize the CENP‐A …

In mammals, CENP-A, a histone H3 variant found in the centromeric chromatin, is critical for
faithful chromosome segregation and genome integrity maintenance through cell divisions …

The kinetochore is an essential structure for chromosome segregation. Although the
kinetochore is usually formed on a centromere locus, it can be artificially formed at a non …