De novo transcriptome assembly: A comprehensive cross-species comparison of short-read RNA-Seq assemblers

M Hölzer, M Marz - Gigascience, 2019 - academic.oup.com
Background In recent years, massively parallel complementary DNA sequencing (RNA
sequencing [RNA-Seq]) has emerged as a fast, cost-effective, and robust technology to …

Transposons contribute to the functional diversification of the head, gut, and ovary transcriptomes across Drosophila natural strains

M Coronado-Zamora, J González - Genome research, 2023 - genome.cshlp.org
Transcriptomes are dynamic, with cells, tissues, and body parts expressing particular sets of
transcripts. Transposable elements (TEs) are a known source of transcriptome diversity; …

ChimeraTE: a pipeline to detect chimeric transcripts derived from genes and transposable elements

DS Oliveira, M Fablet, A Larue, A Vallier… - Nucleic acids …, 2023 - academic.oup.com
Transposable elements (TEs) produce structural variants and are considered an important
source of genetic diversity. Notably, TE-gene fusion transcripts, ie chimeric transcripts, have …

Highly Robust de Novo Full-Length Protein Sequencing

ZB Mai, ZH Zhou, QY He, G Zhang - Analytical chemistry, 2022 - ACS Publications
Accurate full-length sequencing of a purified unknown protein is still challenging nowadays
due to the error-prone mass-spectrometry (MS)-based methods. De novo identified peptide …

[HTML][HTML] The male and female gonad transcriptome of the edible sea urchin, Paracentrotus lividus: Identification of sex-related and lipid biosynthesis genes

AM Machado, S Fernández-Boo, M Nande, R Pinto… - Aquaculture …, 2022 - Elsevier
Paracentrotus lividus is the most abundant, geographically distributed, and desirable
echinoid species in Europe. Although economically important, this species has scarce …

Investigation of the activity of transposable elements and genes involved in their silencing in the newt Cynops orientalis, a species with a giant genome

F Carducci, E Carotti, M Gerdol, S Greco, A Canapa… - Scientific Reports, 2021 - nature.com
Caudata is an order of amphibians with great variation in genome size, which can reach
enormous dimensions in salamanders. In this work, we analysed the activity of transposable …

The Bellerophon pipeline, improving de novo transcriptomes and removing chimeras

J Kerkvliet, A de Fouchier, M van Wijk… - Ecology and …, 2019 - Wiley Online Library
Transcriptome quality control is an important step in RNA‐Seq experiments. However, the
quality of de novo assembled transcriptomes is difficult to assess, due to the lack of …

Doublesex is essential for masculinization but not feminization in Lygus hesperus

JJ Hull, CC Heu, RJ Gross, DM LeRoy… - Insect Biochemistry and …, 2024 - Elsevier
In most holometabolous insects, sex differentiation occurs via a hierarchical cascade of
transcription factors, with doublesex (dsx) regulating genes that control sex-specific traits …

TALC: transcript-level aware long-read correction

L Broseus, A Thomas, AJ Oldfield, D Severac… - …, 2020 - academic.oup.com
Motivation Long-read sequencing technologies are invaluable for determining complex RNA
transcript architectures but are error-prone. Numerous 'hybrid correction'algorithms have …

Compressed weighted de Bruijn graphs

GF Italiano, N Prezza, B Sinaimeri… - CPM 2021-32nd Annual …, 2021 - inria.hal.science
We propose a new compressed representation for weighted de Bruijn graphs, which is
based on the idea of delta-encoding the variations of k-mer abundances on a spanning …