Inteins are internal protein elements that self-excise from their host protein and catalyze
ligation of the flanking sequences (exteins) with a peptide bond. They are found in …

Purification tags are robust tools that can be used to purify a wide selection of target
proteins, which makes them attractive candidates for implementation into platform …

We describe a prokaryotic expression system using the autoproteolytic function of Npro from
classical swine fever virus. Proteins or peptides expressed as Npro fusions are deposited as …

It has been reported that it is difficult to express cationic antibacterial peptides in engineered
bacteria because such peptides are highly toxic to the host bacteria cells and sensitive to …

Protein purification remains a central need for biotechnology. In recent years, a class of
aggregating tags has emerged, which offers a quick, cost‐effective and column‐free …

The purification of recombinant proteins is a complicated process that requires a thorough
understanding of the physical and chemical properties of each protein of interest. The …

About half a century after antibiotics discovery, multi-antibiotic-resistant bacteria posed a
new challenge to medicine. Attempts to discover new antibiotics have drawn the attention to …

Cytochrome P450 BM3 (CYP102A1) from Bacillus megaterium is an interesting target for
biotechnological applications, because of its vast substrate variety combined with high P450 …

In this work, we apply self-cleaving affinity tag technology to several target proteins secreted
into the Escherichia coli periplasm, including two with disulfide bonds. The target proteins …

A dual-vector system was explored for the delivery of the coagulation factor VIII gene, using
intein-mediated protein trans-splicing as a means to produce intact functional factor VIII post …