Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Cell-free protein synthesis (CFPS) is an in vitro process that enables diverse applications in
research, biomanufacturing, point-of-care diagnostics, therapeutics, and education using …

The fragility of biological systems during storage, transport, and utilization necessitates
reliable cold-chain infrastructure and limits the potential of biotechnological applications. In …

Cell-free systems for the in vitro production of proteins have revolutionized the synthetic
biology field. In the last decade, this technology is gaining momentum in molecular biology …

Membrane proteins (MPs) carry out important functions in the metabolism of cells, such as
the detection of extracellular activities and the transport of small molecules across the …

Abstract The National Institute of Standards and Technology (NIST) and the National Center
for Advancing Translational Sciences (NCATS) convened the workshop Advancing Cell-free …

Synthesizing proteins for use in therapeutics is restrained by, in part, contaminants in in vivo
expression systems and limited production capacity of in vitro systems. Cell free expression …