PCR-based method for identifying the S-genotypes of Japanese pear cultivars

T Ishimizu, K Inoue, M Shimonaka, T Saito… - Theoretical and Applied …, 1999 - Springer
Abstract Japanese pear (Pyrus pyrifolia Nakai), a member of the Rosaceae, shows
gametophytic self-incompatibility that is controlled by the S-locus. The S-genotype of …

Cloning of six cherry self-incompatibility alleles and development of allele-specific PCR detection

T Sonneveld, TP Robbins, R Bošković… - Theoretical and Applied …, 2001 - Springer
Reverse transcription of stylar RNA from three cherry cultivars representing six self-
incompatibility (S) alleles, Early Rivers (S 1 S 2), Napoleon (S 3 S 4) and Colney (S 5 S 6) …

Style‐specific self‐compatible mutation caused by deletion of the S‐RNase gene in Japanese pear (Pyrus serotina)

H Sassa, H Hirano, T Nishio, T Koba - The Plant Journal, 1997 - Wiley Online Library
Monofactorial gametophytic self‐incompatibility in the family Rosaceae is controlled by the
single multi‐allelic S‐locus. In Japanese pear, a naturally occurring self‐compatible mutant …

Identification of Stylar RNases Associated with Gametophytic Self-Incompatibility in Almond (Prunus dulcis)

R Tao, H Yamane, H Sassa, H Mori… - Plant and cell …, 1997 - academic.oup.com
Stylar proteins of 13 almond (Prunus dulcis) cultivars with known S-genotypes were
surveyed by IEF and 2D-PAGE combined with immunoblot and N-terminal amino acid …

Primary structural features of rosaceous S-RNases associated with gametophytic self-incompatibility

T Ishimizu, T Shinkawa, F Sakiyama, S Norioka - Plant Molecular Biology, 1998 - Springer
We isolated cDNA clones encoding five S-RNases (S 1-, S3-, S 5-, S 6-, S 7-RNases) from
pistils of Pyrus pyrifolia (Japanese pear), a member of the Rosaceae. Their amino acid …

Identification and Partial Amino Acid Sequences of Seven S-RNases Associated with Self-Incompatibility of Japanese Pear, Pyrus pyrifolia Nakai

T Ishimizu, Y Sato, T Saito, Y Yoshimura… - The journal of …, 1996 - academic.oup.com
S-allele-specific proteins (S-proteins) were separated and identified by two-dimensional
(2D) gel electrophoresis from the style extract of 14 cultivars of Japaneae pear, Pyrus …

[HTML][HTML] Identification of regions in which positive selection may operate in S-RNase of Rosaceae: implication for S-allele-specific recognition sites in S-RNase

T Ishimizu, T Endo, Y Yamaguchi-Kabata… - Febs Letters, 1998 - Elsevier
A stylar S-RNase is associated with gametophytic self-incompatibility in the Rosaceae,
Solanaceae, and Scrophulariaceae. This S-RNase is responsible for S-allele-specific …

Molecular cloning and nucleotide sequences of cDNAs encoding S-allele specific stylar RNases in a self-incompatible cultivar and its self-compatible mutant of …

N Norioka, S Norioka, Y Ohnishi… - The journal of …, 1996 - academic.oup.com
The genes encoding three RNases were cloned from the style of a self-incompatible cultivar,
Nijisseiki (S2S4), and its self-compatible mutant, Osa-Nijisseiki (S2S4sm, sm means stylar …

[HTML][HTML] Determination of S-genotypes of pear (Pyrus pyrifolia) cultivars by S-RNase sequencing and PCR-RFLP analyses

HT Kim, Y Hirata, IS Nou - Molecules and cells, 2002 - Elsevier
The pear (Pyrus pyrifolia) has gametophytic self-incompatibility (GSI). To elucidate the S-
genotypes of Korean-bred pear cultivars, whose parents are heterozygotes, the PCR …

Characteristic of pollen tube that grew into self style in pear cultivar and parent assignment for cross-pollination

GM Wang, C Gu, X Qiao, BY Zhao, YQ Ke, BB Guo… - Scientia …, 2017 - Elsevier
Pear is one of the most important fruits in the world. In recent years, many new varieties have
been bred. However, little is known about these new varieties, including pollen germination …