The Wnt pathway is central to a host of developmental and disease-related processes. The
remarkable conservation of this intercellular signaling cascade throughout metazoan …

The genetically encoded fluorescent sensors convert chemical and physical signals into
light. They are powerful tools for the visualisation of physiological processes in living cells …

Highlights•Self-contained overview of current high-throughput screening technologies.•
Expands on the current use of most recent tools, like high-throughput microscopy.•Learn …

Fluorescence resonance energy transfer (FRET)-based biosensors are effective analytical
tools extensively used in fields of biomedicine, pharmacology, toxicology, and food …

The purpose of this work is to provide an exhaustive overview of the emerging biosensor
technologies for the detection of analytes of interest for food, environment, security, and …

The understanding of signaling and metabolic processes in multicellular organisms requires
knowledge of the spatial dynamics of small molecules and the activities of enzymes …

Bioactive peptides, specific protein fragments with positive health effects, are gaining
traction in drug development for advantages like enhanced penetration, low toxicity, and …

Green fluorescent proteins (GFPs) have become indispensable imaging and optogenetic
tools. Their absorption and emission properties can be optimized for specific applications …

Genetically encoded biosensors based on fluorescent proteins (FPs) allow for the real-time
monitoring of molecular dynamics in space and time, which are crucial for the proper …

Splitting bioactive proteins into conditionally reconstituting fragments is a powerful strategy
for building tools to study and control biological systems. However, split proteins often …