Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

M Ahmad, M Hirz, H Pichler, H Schwab - Applied microbiology and …, 2014 - Springer
Pichia pastoris is an established protein expression host mainly applied for the production of
biopharmaceuticals and industrial enzymes. This methylotrophic yeast is a distinguished …

An updated view on horseradish peroxidases: recombinant production and biotechnological applications

FW Krainer, A Glieder - Applied microbiology and biotechnology, 2015 - Springer
Horseradish peroxidase has been the subject of scientific research for centuries. It has been
used exhaustively as reporter enzyme in diagnostics and histochemistry and still plays a …

Regulation of Pichia pastoris promoters and its consequences for protein production

T Vogl, A Glieder - New biotechnology, 2013 - Elsevier
The methylotrophic yeast Pichia pastoris is a widely used host for heterologous protein
production. Along with favorable properties such as growth to high cell density and high …

Protein secretion in Pichia pastoris and advances in protein production

LM Damasceno, CJ Huang, CA Batt - Applied microbiology and …, 2012 - Springer
Yeast expression systems have been successfully used for over 20 years for the production
of recombinant proteins. With the growing interest in recombinant protein expression for …

Engineering of the unfolded protein response pathway in Pichia pastoris: enhancing production of secreted recombinant proteins

H Raschmanová, A Weninger, Z Knejzlík… - Applied Microbiology …, 2021 - Springer
Folding and processing of proteins in the endoplasmic reticulum (ER) are major
impediments in the production and secretion of proteins from Pichia pastoris (Komagataella …

Pichia Pastoris: Protein Production Host and Model Organism for Biomedical Research

B Gasser, R Prielhofer, H Marx, M Maurer… - Future …, 2013 - Taylor & Francis
Pichia pastoris is the most frequently used yeast system for heterologous protein production
today. The last few years have seen several products based on this platform reach approval …

Yeast synthetic biology for the production of recombinant therapeutic proteins

H Kim, SJ Yoo, HA Kang - FEMS yeast research, 2015 - scholarworks.bwise.kr
The production of recombinant therapeutic proteins is one of the fast-growing areas of
molecular medicine and currently plays an important role in treatment of several diseases …

Towards systems metabolic engineering in Pichia pastoris

JP Schwarzhans, T Luttermann, M Geier… - Biotechnology …, 2017 - Elsevier
The methylotrophic yeast Pichia pastoris is firmly established as a host for the production of
recombinant proteins, frequently outperforming other heterologous hosts. Already, a …

Systems biotechnology for protein production in Pichia pastoris

RJ Zahrl, DA Peña, D Mattanovich… - FEMS yeast …, 2017 - academic.oup.com
The methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) is one of the most
important production systems for heterologous proteins. After the first genome sequences …

Expression of enzymes for the usage in food and feed industry with Pichia pastoris

SC Spohner, H Müller, H Quitmann, P Czermak - Journal of Biotechnology, 2015 - Elsevier
The methylotrophic yeast Pichia pastoris is an established protein expression host for the
production of industrial enzymes. This yeast can be grown to very high cell densities and …