Carbohydrate esterases (CEs) catalyze the de-O or de-N-acylation by removing the ester
decorations from carbohydrates. CEs are currently classified in 15 families in the …

Acetyl and methyl esterifications are two major naturally found substitutions in the plant cell-
wall polysaccharides. The non-cellulosic plant cell-wall polysaccharides such as pectin and …

Biocatalysts exerting activity against ester bonds have a broad range of applications in
modern biotechnology. Here, we have identified a new esterolytic enzyme by screening a …

Pneumococcal neuraminidase is a key enzyme for sequential deglycosylation of host
glycans, and plays an important role in host survival, colonization, and pathogenesis of …

A pH-sensitive colorimetric assay has been established to quantitatively measure glutamate
decarboxylase (GAD) activity in bacterial cell extracts using a microplate format. GAD …

Cross-linked enzyme aggregates (CLEAs) are reported for the first time for a recombinant
acetyl xylan esterase (AXE) from Bacillus pumilus. With this enzyme, CLEAs production was …

Protein engineering using directed evolution or saturation mutagenesis at hot spots is often
used to improve enzyme properties such as their substrate selectivity or stability. This …

TM0077 from Thermotoga maritima is a member of the carbohydrate esterase family 7 and is
active on a variety of acetylated compounds, including cephalosporin C. TM0077 esterase …

Acetyl esterases are an important component of the enzymatic machinery fungi use to
degrade plant biomass and are classified in several Carbohydrate Esterase families of the …

Cold-active acetyl xylan esterases allow for reduced bioreactor heating costs in bioenergy
production. Here, we isolated and characterized a cold-active acetyl xylan esterase (Pb AcE) …