Expression-enhanced fluorescent proteins based on enhanced green fluorescent protein for super-resolution microscopy

S Duwé, E De Zitter, V Gielen, B Moeyaert… - ACS …, 2015 - ACS Publications
“Smart fluorophores”, such as reversibly switchable fluorescent proteins, are crucial for
advanced fluorescence imaging. However, only a limited number of such labels is available …

Green-to-red photoconvertible Dronpa mutant for multimodal super-resolution fluorescence microscopy

B Moeyaert, N Nguyen Bich, E De Zitter, S Rocha… - ACS …, 2014 - ACS Publications
Advanced imaging techniques crucially depend on the labels used. In this work, we present
the structure-guided design of a fluorescent protein that displays both reversibly …

It's a two‐way street: Photoswitching and reversible changes of the protein matrix in photoswitchable fluorescent proteins and bacteriophytochromes

EC Rodrigues, AC Stiel - FEBS letters, 2023 - Wiley Online Library
Chromophore‐bearing proteins that are (reversibly) altered after light illumination are major
functional components of nature. They gained considerable attention in the last decades …

Phototransformable fluorescent proteins: which one for which application?

V Adam - Histochemistry and cell biology, 2014 - Springer
In these last two decades, fluorescent proteins (FPs) have become highly valued imaging
tools for cell biology, owing to their compatibility with living samples, their low levels of …

Time and frequency-domain measurement of ground-state recovery times in red fluorescent proteins

P Manna, R Jimenez - The Journal of Physical Chemistry B, 2015 - ACS Publications
The field of bioimaging and biosensors has been revolutionized by the discovery of
fluorescent proteins (FPs) and their use in live cells. FPs are characterized with rich …

Structural basis of photoswitching in fluorescent proteins

C Duan, V Adam, M Byrdin, D Bourgeois - … Proteins: Methods and …, 2014 - Springer
Fluorescent proteins have revolutionized life sciences because they allow noninvasive and
highly specific labeling of biological samples. The subset of “phototransformable” …

Reversibly switchable fluorescent proteins:“the fair switch project”

R Nifosì, B Storti, R Bizzarri - La Rivista del Nuovo Cimento, 2024 - Springer
Fluorescent proteins (FPs) have transformed cell biology through their use in fluorescence
microscopy, enabling precise labeling of proteins via genetic fusion. A key advancement is …

An unusual disulfide-linked dimerization in the fluorescent protein rsCherryRev1. 4

TYH Bui, P Dedecker… - … Crystallographica Section F …, 2023 - journals.iucr.org
rsCherryRev1. 4 has been reported as one of the reversibly photoswitchable variants of
mCherry, and is an improved version with a faster off-switching speed and lower switching …

Room temperature crystal structure of the fast switching M159T mutant of the fluorescent protein dronpa

M Kaucikas, A Fitzpatrick, E Bryan… - Proteins: Structure …, 2015 - Wiley Online Library
The fluorescent protein Dronpa undergoes reversible photoswitching reactions between the
bright “on” and dark “off” states via photoisomerization and proton transfer reactions. We …

Development and Characterization of Improved Red Fluorescent Protein Variants

P Manna - 2017 - search.proquest.com
Aequorea victoria-based green fluorescent proteins and their blue, cyan and red
counterparts offer unprecedented advantage as biological markers owing to their genetic …