Engineering mammalian cells to carry out sophisticated and customizable genetic programs
requires a toolkit of multiple orthogonal and well-characterized transcription factors (TFs). To …

Synthetic receptors are powerful tools for engineering mammalian cell-based devices.
These biosensors enable cell-based therapies to perform complex tasks such as regulating …

Cellular signaling is controlled by ligand receptor interaction and subsequent biochemical
changes inside the cell. Manipulating receptors as per need that can be a strategy to alter …

Towards the goal of making 'smart'cell therapies, one that recognizes disease conditions (eg
hypoxia) and then produces mitigating biologics, it is important to develop suitable …

Technologies for engineering immune cells to recognize features on cancer cells are
transforming oncology. Synthetic biologists now expand this approach by conferring …

Living cells can be engineered into cell-based therapeutics by utilizing synthetic genetic
elements and protein effectors that reprogram cell activity to be able to treat diseases …

Cell engineering presents great potential for next-generation biotechnological and
biomedical applications that employ engineered mammalian cells to sense intracellular or …

This dissertation focuses on the development of quantitative approaches for characterizing
endogenous signaling pathways and designing new pathways in mammalian cells. I …