Applications and advances in molecular diagnostics: revolutionizing non-tuberculous mycobacteria species and subspecies identification

H Zhang, M Tang, D Li, M Xu, Y Ao, L Lin - Frontiers in Public Health, 2024 - frontiersin.org
Non-tuberculous mycobacteria (NTM) infections pose a significant public health challenge
worldwide, affecting individuals across a wide spectrum of immune statuses. Recent …

Culture, identification, and antimicrobial susceptibility testing of pulmonary nontuberculous mycobacteria

R Khare, BA Brown-Elliott - Clinics in Chest Medicine, 2023 - chestmed.theclinics.com
BACKGROUND Nontuberculous mycobacterial (NTM) pulmonary disease is increasing
globally. 1 Pulmonary infections are typically opportunistic and associated with immune …

Multicentre study on the reproducibility of MALDI-TOF MS for nontuberculous mycobacteria identification

D Rodriguez-Temporal, F Alcaide, I Mareković… - Scientific reports, 2022 - nature.com
The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has
improved recently thanks to updated databases and optimized protein extraction …

Performance evaluation and clinical validation of optimized nucleotide MALDI-TOF-MS for mycobacterial identification

B Li, C Zhu, L Sun, H Dong, Y Sun, S Cao… - Frontiers in Cellular …, 2022 - frontiersin.org
Objective To evaluate the performance and validate the diagnostic value of a nucleotide
matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS) with the …

Identification of Mycobacterium abscessus subspecies by MALDI-TOF mass spectrometry and machine learning

D Rodríguez-Temporal, L Herrera… - Journal of clinical …, 2023 - Am Soc Microbiol
Mycobacterium abscessus is one of the most common and pathogenic nontuberculous
mycobacteria (NTM) isolated in clinical laboratories. It consists of three subspecies: M …

Evaluation of two different Preparation protocols for MALDI-TOF MS nontuberculous mycobacteria identification from Liquid and Solid Media

L Pastrone, A Curtoni, G Criscione, F Scaiola, P Bottino… - Microorganisms, 2023 - mdpi.com
Nontuberculous mycobacteria (NTM) identification is essential for establishing the relevance
of the isolate and for appropriate antimicrobial therapy. Traditionally, NTM identification is …

Evaluation of MALDI Biotyper Mycobacteria library for identification of nontuberculous mycobacteria

NC Toney, W Zhu, B Jensen, J Gartin… - Journal of Clinical …, 2022 - Am Soc Microbiol
ABSTRACT The Bruker Biotyper matrix-assisted laser desorption ionization–time-of-flight
mass spectrometry (MALDI-TOF MS) platform was assessed on its ability to accurately …

Rapid and accurate identification of nontuberculous mycobacteria directly from positive primary MGIT cultures by MALDI-TOF MS

L Rindi, V Puglisi, I Franconi, R Fais, A Lupetti - Microorganisms, 2022 - mdpi.com
Over the last years, nontuberculous mycobacteria (NTM) have emerged as important human
pathogens. Accurate and rapid mycobacterial species identification is needed to …

基质辅助激光解吸电离飞行时间质谱分析技术在微生物鉴定与分型中的应用.

张秋艳, 唐静, 祝素珍, 贾俊涛… - Journal of Food …, 2021 - search.ebscohost.com
微生物的鉴定方法有很多种, 这些方法基于表型, 免疫学, 遗传学等, 目前普遍采用16S rRNA
和18S rRNA 基因测序为标准, 但这些方法也有一些局限性, 费时费力, 操作复杂 …

A novel time-lapse imaging method for studying developing bacterial biofilms

M Futo, T Široki, S Koska, N Čorak, A Tušar… - Scientific reports, 2022 - nature.com
In nature, bacteria prevailingly reside in the form of biofilms. These elaborately organized
surface-bound assemblages of bacterial cells show numerous features of multicellular …