Are we there yet? Assessing the readiness of single-cell proteomics to answer biological hypotheses

AA Nitz, JH Giraldez Chavez, ZG Eliason… - Journal of Proteome …, 2024 - ACS Publications
Single-cell analysis is an active area of research in many fields of biology. Measurements at
single-cell resolution allow researchers to study diverse populations without losing …

The Future of Proteomics is Up in the Air: Can Ion Mobility Replace Liquid Chromatography for High Throughput Proteomics?

Y Jiang, D DeBord, H Vitrac, J Stewart… - Journal of Proteome …, 2024 - ACS Publications
The coevolution of liquid chromatography (LC) with mass spectrometry (MS) has shaped
contemporary proteomics. LC hyphenated to MS now enables quantification of more than …

Deep Learning Predicts Non-Normal Transmission Distributions in High-Field Asymmetric Waveform Ion Mobility (FAIMS) Directly from Peptide Sequence

J McKetney, IJ Miller, A Hutton, P Sinitcyn… - Analytical …, 2025 - ACS Publications
Peptide ion mobility adds an extra dimension of separation to mass spectrometry-based
proteomics. The ability to accurately predict peptide ion mobility would be useful to expedite …

Intact protein barcoding enables one-shot identification of CRISPRi strains and their metabolic state

V Pahl, P Lubrano, F Troßmann, D Petras, H Link - Cell Reports Methods, 2024 - cell.com
Detecting strain-specific barcodes with mass spectrometry can facilitate the screening of
genetically engineered bacterial libraries. Here, we introduce intact protein barcoding, a …

Comprehensive overview of bottom-up proteomics using mass spectrometry

Y Jiang, DAB Rex, D Schuster, BA Neely… - ACS Measurement …, 2024 - ACS Publications
Proteomics is the large scale study of protein structure and function from biological systems
through protein identification and quantification.“Shotgun proteomics” or “bottom-up …