Our knowledge of gene function has increasingly lagged behind gene discovery, hindering
our understanding of the genetic basis of microbial phenotypes. Recently, however …

Mycobacterium tuberculosis, which is the aetiological agent of tuberculosis, owes much of its
success as a pathogen to its unique cell wall and unusual mechanism of growth, which …

Antibacterial agents target the products of essential genes but rarely achieve complete
target inhibition. Thus, the all-or-none definition of essentiality afforded by traditional genetic …

For decades, identifying the regions of a bacterial chromosome that are necessary for
viability has relied on mapping integration sites in libraries of random transposon mutants to …

Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …

The development of new drug regimens that allow rapid, sterilizing treatment of tuberculosis
has been limited by the complexity and time required for genetic manipulations in …

Transposon-directed insertion site sequencing (TraDIS) is a high-throughput method
coupling transposon mutagenesis with short-fragment DNA sequencing. It is commonly used …

Generalist and specialist species differ in the breadth of their ecological niches. Little is
known about the niche width of obligate human pathogens. Here we analyzed a global …

The combination of high-density transposon-mediated mutagenesis and high-throughput
sequencing has led to significant advancements in research on essential genes, resulting in …

Bacteria that cause disease rely on their ability to counteract and overcome host defenses.
Here, we present a genome-scale study of Mycobacterium tuberculosis (Mtb) that uncovers …