Transcriptomics studies often rely on partial reference transcriptomes that fail to capture the
full catalogue of transcripts and their variations. Recent advances in sequencing …

A comprehensive understanding of host–pathogen interactions requires a knowledge of the
associated gene expression changes in both the pathogen and the host. Traditional, probe …

New normal linear modeling strategies are presented for analyzing read counts from RNA-
seq experiments. The voom method estimates the mean-variance relationship of the log …

Recent advances in high-throughput RNA sequencing (RNA-seq) have enabled
tremendous leaps forward in our understanding of bacterial transcriptomes. However …

The molecular roles of many RNA‐binding proteins in bacterial post‐transcriptional gene
regulation are not well understood. Approaches combining in vivo UV crosslinking with RNA …

Understanding RNA processing and turnover requires knowledge of cleavages by major
endoribonucleases within a living cell. We have employed TIER-seq (transiently inactivating …

Background Next generation sequencing is transforming our understanding of
transcriptomes. It can determine the expression level of transcripts with a dynamic range of …

Salmonella enterica serovar Typhi, the cause of typhoid, is host restricted to humans. S.
Typhi has a monophyletic population structure, indicating that typhoid in humans is a …

Next-generation sequencing technologies are now being exploited not only to analyse static
genomes, but also dynamic transcriptomes in an approach termed RNA-seq. Although these …

Dietary polyphenols are components of many foods such as tea, fruit, and vegetables and
are associated with several beneficial health effects although, so far, largely based on …