Nanobodies: chemical functionalization strategies and intracellular applications

D Schumacher, J Helma, AFL Schneider… - Angewandte Chemie …, 2018 - Wiley Online Library
Nanobodies can be seen as next‐generation tools for the recognition and modulation of
antigens that are inaccessible to conventional antibodies. Due to their compact structure and …

Nanobodies and recombinant binders in cell biology

J Helma, MC Cardoso, S Muyldermans… - Journal of Cell …, 2015 - rupress.org
Antibodies are key reagents to investigate cellular processes. The development of
recombinant antibodies and binders derived from natural protein scaffolds has expanded …

Proteomics-based methods for discovery, quantification, and validation of protein–protein interactions

YV Miteva, HG Budayeva, IM Cristea - Analytical chemistry, 2013 - ACS Publications
Proteins play a fundamental role in establishing the diversity of cellular processes in health
or disease systems. This diversity is accomplished by a vast array of protein functions. In …

Mutations in ILK, encoding integrin-linked kinase, are associated with arrhythmogenic cardiomyopathy

A Brodehl, S Rezazadeh, T Williams, NM Munsie… - Translational …, 2019 - Elsevier
Arrhythmogenic cardiomyopathy is a genetic heart muscle disorder characterized by fibro-
fatty replacement of cardiomyocytes leading to life-threatening ventricular arrhythmias, heart …

Binding of MBD proteins to DNA blocks Tet1 function thereby modulating transcriptional noise

AK Ludwig, P Zhang, FD Hastert, S Meyer… - Nucleic Acids …, 2017 - academic.oup.com
Aberrant DNA methylation is a hallmark of various human disorders, indicating that the
spatial and temporal regulation of methylation readers and modifiers is imperative for …

Direct homo-and hetero-interactions of MeCP2 and MBD2

A Becker, L Allmann, M Hofstätter, V Casà, P Weber… - PLoS …, 2013 - journals.plos.org
Epigenetic marks like methylation of cytosines at CpG dinucleotides are essential for
mammalian development and play a major role in the regulation of gene expression and …

Touch, act and go: landing and operating on nucleosomes

V Speranzini, S Pilotto, TK Sixma, A Mattevi - The EMBO journal, 2016 - embopress.org
Chromatin‐associated enzymes are responsible for the installation, removal and reading of
precise post‐translation modifications on DNA and histone proteins. They are specifically …

Nanobodys: Strategien zur chemischen Funktionalisierung und intrazelluläre Anwendungen

D Schumacher, J Helma, AFL Schneider… - Angewandte …, 2018 - Wiley Online Library
Nanobodys sind ein modernes Mittel zur Erkennung und Modulation von Antigenen, die für
herkömmliche Antikörper unzugänglich sind. Aufgrund ihrer kompakten Form und ihrer …

Camelid reporter gene imaging: a generic method for in vivo cell tracking

LRY Goethals, TJ Bos, L Baeyens, F De Geeter… - EJNMMI research, 2014 - Springer
Background To combine the sensitivity of bioluminescent imaging (BLI) with the 3D and
quantitative properties of pinhole single-photon emission computed tomography …

Estimation of interaction between oriented immobilized green fluorescent protein and its antibody by high performance affinity chromatography and molecular docking

Q Li, J Wang, L Yang, X Gao, H Chen… - Journal of Molecular …, 2015 - Wiley Online Library
Although green fluorescence protein (GFP) and its antibody are widely used to track a
protein or a cell in life sciences, the binding behavior between them remains unclear. In this …