Promoter Architecture and Promoter Engineering in Saccharomyces cerevisiae
Promoters play an essential role in the regulation of gene expression for fine-tuning genetic
circuits and metabolic pathways in Saccharomyces cerevisiae (S. cerevisiae). However …
circuits and metabolic pathways in Saccharomyces cerevisiae (S. cerevisiae). However …
MitoCPR—A surveillance pathway that protects mitochondria in response to protein import stress
H Weidberg, A Amon - Science, 2018 - science.org
INTRODUCTION Mitochondria provide cells with energy and numerous essential
metabolites such as lipids, amino acids, iron sulfur clusters, and heme. All mitochondrial …
metabolites such as lipids, amino acids, iron sulfur clusters, and heme. All mitochondrial …
Functional annotation of chemical libraries across diverse biological processes
JS Piotrowski, SC Li, R Deshpande… - Nature Chemical …, 2017 - nature.com
Chemical-genetic approaches offer the potential for unbiased functional annotation of
chemical libraries. Mutations can alter the response of cells in the presence of a compound …
chemical libraries. Mutations can alter the response of cells in the presence of a compound …
Maturation-driven transport and AP-1–dependent recycling of a secretory cargo in the Golgi
Golgi cisternal maturation has been visualized by fluorescence imaging of individual
cisternae in the yeast Saccharomyces cerevisiae, but those experiments did not track …
cisternae in the yeast Saccharomyces cerevisiae, but those experiments did not track …
Identification of Essential Genes and Fluconazole Susceptibility Genes in Candida glabrata by Profiling Hermes Transposon Insertions
AN Gale, RM Sakhawala, A Levitan… - G3: Genes …, 2020 - academic.oup.com
Within the budding yeasts, the opportunistic pathogen Candida glabrata and other members
of the Nakaseomyces clade have developed virulence traits independently from C. albicans …
of the Nakaseomyces clade have developed virulence traits independently from C. albicans …
A microscopy-based kinetic analysis of yeast vacuolar protein sorting
Saccharomyces cerevisiae is amenable to studying membrane traffic by live-cell
fluorescence microscopy. We used this system to explore two aspects of cargo protein traffic …
fluorescence microscopy. We used this system to explore two aspects of cargo protein traffic …
Enhanced astaxanthin production in S. cerevisiae by combinatorial engineering of gene targets outside the synthetic pathway
S Mao, H Yu, L Ye - Biochemical Engineering Journal, 2023 - Elsevier
In recent years, microbial synthesis of astaxanthin with strong antioxidant activity has
received widespread attention. In addition to the extensively engineered genes within the …
received widespread attention. In addition to the extensively engineered genes within the …
ESCargo: a regulatable fluorescent secretory cargo for diverse model organisms
Membrane traffic can be studied by imaging a cargo protein as it transits the secretory
pathway. The best tools for this purpose initially block export of the secretory cargo from the …
pathway. The best tools for this purpose initially block export of the secretory cargo from the …
An improved reversibly dimerizing mutant of the FK506-binding protein FKBP
JJ Barrero, E Papanikou, JC Casler, KJ Day… - Cellular …, 2016 - Taylor & Francis
ABSTRACT FK506-binding protein (FKBP) is a monomer that binds to FK506, rapamycin,
and related ligands. The F36M substitution, in which Phe36 in the ligand-binding pocket is …
and related ligands. The F36M substitution, in which Phe36 in the ligand-binding pocket is …
Genomic and targeted approaches unveil the cell membrane as a major target of the antifungal cytotoxin amantelide A
LA Elsadek, JH Matthews, S Nishimura… - …, 2021 - Wiley Online Library
Amantelide A, a polyhydroxylated macrolide isolated from a marine cyanobacterium,
displays broad‐spectrum activity against mammalian cells, bacterial pathogens, and marine …
displays broad‐spectrum activity against mammalian cells, bacterial pathogens, and marine …