Onsite detection of plant viruses using isothermal amplification assays
Plant diseases caused by viruses limit crop production and quality, resulting in significant
losses. However, options for managing viruses are limited; for example, as systemic obligate …
losses. However, options for managing viruses are limited; for example, as systemic obligate …
Emerging and re-emerging diseases caused by Badnaviruses
A Ishwara Bhat, R Selvarajan, V Balasubramanian - Pathogens, 2023 - mdpi.com
New and emerging plant diseases are caused by different pathogens including viruses that
often cause significant crop losses. Badnaviruses are pararetroviruses that contain a single …
often cause significant crop losses. Badnaviruses are pararetroviruses that contain a single …
Development of a recombinase polymerase based isothermal amplification combined with lateral flow assay (HLB-RPA-LFA) for rapid detection of "Candidatus …
Huanglongbing (HLB) or citrus greening is highly destructive disease that is affecting the
citrus industry worldwide and it has killed millions of citrus plants globally. HLB is caused by …
citrus industry worldwide and it has killed millions of citrus plants globally. HLB is caused by …
Development of a reverse transcription recombinase polymerase based isothermal amplification coupled with lateral flow immunochromatographic assay (CTV-RT …
Tristeza is a highly destructive disease of citrus caused by the phloem-limited, flexuous
filamentous Citrus tristeza virus (CTV) in the genus Closterovirus and the family …
filamentous Citrus tristeza virus (CTV) in the genus Closterovirus and the family …
Simple template-based reverse transcription-recombinase polymerase amplification assay for routine diagnosis of citrus tristeza virus
SK Sharma, N Pathaw, B Wangkhem… - Letters in Applied …, 2023 - academic.oup.com
This study reports a simple template-based reverse transcription-polymerase amplification
assay (ST-RT-RPA) for detection of citrus tristeza virus (CTV) from crude plant extract lysed …
assay (ST-RT-RPA) for detection of citrus tristeza virus (CTV) from crude plant extract lysed …
Development and application of crude sap-based recombinase polymerase amplification assay for the detection and occurrence of grapevine geminivirus A in Indian …
Geminiviruses are known to infect several fields and horticultural crops around the globe.
Grapevine geminivirus A (GGVA) was reported in the United States in 2017, and since then …
Grapevine geminivirus A (GGVA) was reported in the United States in 2017, and since then …
Recent Developments in Applied Microbiology and Biochemistry: Volume 2
V Buddolla - 2020 - books.google.com
Recent Developments in Applied Microbiology and Biochemistry, Vol. 2, provides a
comprehensive treatment and understanding on application oriented microbial concepts …
comprehensive treatment and understanding on application oriented microbial concepts …
Rapid and sensitive detection of potato virus Y by isothermal reverse transcription-recombinase polymerase amplification assay in potato
Y Wang, R Chen, X Nie, Z Zhong, C Li, K Li… - Molecular and cellular …, 2020 - Elsevier
In this study, an isothermal reverse transcription-recombinase polymerase amplification (RT-
RPA) assay was developed for the efficient and accurate detection of potato virus Y (PVY) …
RPA) assay was developed for the efficient and accurate detection of potato virus Y (PVY) …
重组酶聚合酶扩增技术在植物病毒检测中的应用
罗雪琮， 安梦楠， 吴元华， 夏子豪 - 生物技术通报, 2022 - biotech.aiijournal.com
随着分子生物学技术的发展, 多种核酸等温扩增技术逐渐被开发出来. 其中, 重组酶聚合酶扩增(
recombinase polymerase amplification, RPA) 作为一种快速, 灵敏的检测技术具有很大的优势 …
recombinase polymerase amplification, RPA) 作为一种快速, 灵敏的检测技术具有很大的优势 …
RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors
DNA extraction can be lengthy and sometimes ends up with amplification inhibitors. We
present the potential of recombinase polymerase amplification (RPA) to replace plant DNA …
present the potential of recombinase polymerase amplification (RPA) to replace plant DNA …