Moulding the mould: understanding and reprogramming filamentous fungal growth and morphogenesis for next generation cell factories

TC Cairns, X Zheng, P Zheng, J Sun, V Meyer - Biotechnology for Biofuels, 2019 - Springer
Filamentous fungi are harnessed as cell factories for the production of a diverse range of
organic acids, proteins, and secondary metabolites. Growth and morphology have critical …

Detection technologies in proteome analysis

WF Patton - Journal of Chromatography B, 2002 - Elsevier
Common strategies employed for general protein detection include organic dye, silver stain,
radiolabeling, reverse stain, fluorescent stain, chemiluminescent stain and mass …

Fungal biotechnology

JL Adrio, AL Demain - International Microbiology, 2003 - Springer
Fungi are used in many industrial processes, such as the production of enzymes, vitamins,
polysaccharides, polyhydric alcohols, pigments, lipids, and glycolipids. Some of these …

The secretion pathway in filamentous fungi: a biotechnological view

A Conesa, PJ Punt, N van Luijk… - Fungal Genetics and …, 2001 - Elsevier
The high capacity of the secretion machinery of filamentous fungi has been widely exploited
for the production of homologous and heterologous proteins; however, our knowledge of the …

Expression and export: recombinant protein production systems for Aspergillus

A Fleißner, P Dersch - Applied microbiology and biotechnology, 2010 - Springer
Several Aspergillus species, in particular Aspergillus niger and Aspergillus oryzae, are
widely used as protein production hosts in various biotechnological applications. In order to …

Phenotypic alteration of eukaryotic cells using randomized libraries of artificial transcription factors

KS Park, D Lee, H Lee, Y Lee, YS Jang, YH Kim… - Nature …, 2003 - nature.com
We have developed a method in which randomized libraries of zinc finger–containing
artificial transcription factors are used to induce phenotypic variations in yeast and …

Filamentous fungi as microbial cell factories for food use

DB Archer - Current opinion in biotechnology, 2000 - Elsevier
The knowledge base that will underpin the more efficient use of filamentous fungi as cell
factories in food has increased during the past year in the areas of gene regulation, protein …

Studies on the Production of Fungal Peroxidases inAspergillus niger

A Conesa, CA van den Hondel… - Applied and …, 2000 - Am Soc Microbiol
To get insight into the limiting factors existing for the efficient production of fungal peroxidase
in filamentous fungi, the expression of the Phanerochaete chrysosporium lignin peroxidase …

Expression of the Pycnoporus cinnabarinus laccase gene in Aspergillus niger and characterization of the recombinant enzyme

E Record, PJ Punt, M Chamkha, M Labat… - European Journal of …, 2002 - Wiley Online Library
Pycnoporus cinnabarinus laccase lac1 gene was overexpressed in Aspergillus niger, a well‐
known fungal host producing a large amount of homologous or heterologous enzymes for …

Something old, something new: challenges and developments in Aspergillus niger biotechnology

TC Cairns, L Barthel, V Meyer - Essays in Biochemistry, 2021 - portlandpress.com
The filamentous ascomycete fungus Aspergillus niger is a prolific secretor of organic acids,
proteins, enzymes and secondary metabolites. Throughout the last century, biotechnologists …