Differences in gene sequences, many of which are single nucleotide polymorphisms,
underlie some of the most important traits in plants. With humanity facing significant …

Premature termination codons (PTCs) are equivalent to nonsense sequences. They encode
no amino acid, and their presence precludes the synthesis of full-length proteins …

Here, we report a form of oligonucleotide-directed mutagenesis for precision genome editing
in plants that uses single-stranded oligonucleotides (ssODN s) to precisely and efficiently …

Single-stranded DNA oligonucleotides (ssODNs) can direct the repair of a single base
mutation in human genes. While the regulation of this gene editing reaction has been …

CRISPR/Cas9 and single-stranded DNA oligonucleotides (ssODNs) have been used to
direct the repair of a single base mutation in human genes. Here, we examine a method …

The capacity to correct a mutant gene within the context of the chromosome holds great
promise as a therapy for inherited disorders but fulfilling this promise has proven to be …

It is now well established that in yeast, and likely most eukaryotic organisms, initial DNA
replication of the leading strand is by DNA polymerase ε and of the lagging strand by DNA …

The last few years have seen tremendous advances in CRISPR-mediated genome editing.
Great efforts have been made to improve the efficiency, specificity, editing window, and …

Background Targeted gene repair is an attractive method to correct point‐mutated genes at
their natural chromosomal sites, but it is still rather inefficient. As revealed by earlier studies …

Abstract PolyPurine Reverse Hoogsteen hairpins (PPRHs) are DNA hairpins formed by
intramolecular reverse Hoogsteen bonds which can bind to polypyrimidine stretches in …