Viruses defined by the position of the virosphere within the replicator space

EV Koonin, VV Dolja, M Krupovic… - … and Molecular Biology …, 2021 - Am Soc Microbiol
Originally, viruses were defined as miniscule infectious agents that passed through filters
that retain even the smallest cells. Subsequently, viruses were considered obligate …

Next-generation sequencing and genome editing in plant virology

A Hadidi, R Flores, T Candresse, M Barba - Frontiers in microbiology, 2016 - frontiersin.org
Next-generation sequencing (NGS) has been applied to plant virology since 2009. NGS
provides highly efficient, rapid, low cost DNA, or RNA high-throughput sequencing of the …

[HTML][HTML] Are viruses alive? The replicator paradigm sheds decisive light on an old but misguided question

EV Koonin, P Starokadomskyy - Studies in history and philosophy of …, 2016 - Elsevier
The question whether or not “viruses are alive” has caused considerable debate over many
years. Yet, the question is effectively without substance because the answer depends …

Novel ribozymes: discovery, catalytic mechanisms, and the quest to understand biological function

CE Weinberg, Z Weinberg… - Nucleic acids research, 2019 - academic.oup.com
Small endonucleolytic ribozymes promote the self-cleavage of their own phosphodiester
backbone at a specific linkage. The structures of and the reactions catalysed by members of …

Plant virology delivers diverse toolsets for biotechnology

M Wang, S Gao, W Zeng, Y Yang, J Ma, Y Wang - Viruses, 2020 - mdpi.com
Over a hundred years of research on plant viruses has led to a detailed understanding of
viral replication, movement, and host–virus interactions. The functions of vast viral genes …

Symptomatic plant viroid infections in phytopathogenic fungi

S Wei, R Bian, IB Andika, E Niu, Q Liu… - Proceedings of the …, 2019 - National Acad Sciences
Viroids are pathogenic agents that have a small, circular noncoding RNA genome. They
have been found only in plant species; therefore, their infectivity and pathogenicity in other …

Global Transcriptomic Changes Induced by Infection of Cucumber (Cucumis sativus L.) with Mild and Severe Variants of Hop Stunt Viroid

C Xia, S Li, W Hou, Z Fan, H Xiao, M Lu… - Frontiers in …, 2017 - frontiersin.org
Fifteen years after transfer to hops, hop stunt viroid-grapevine (HSVd-g) was replaced by
HSVd-hop (HSVd-h), a sequence variant that contains changes at five different positions …

Viroids and viroid-like circular RNAs: Do they descend from primordial replicators?

BD Lee, EV Koonin - Life, 2022 - mdpi.com
Viroids are a unique class of plant pathogens that consist of small circular RNA molecules,
between 220 and 450 nucleotides in size. Viroids encode no proteins and are the smallest …

Hop stunt viroid: A polyphagous pathogenic RNA that has shed light on viroid–host interactions

J Marquez‐Molins, G Gomez… - Molecular Plant …, 2021 - Wiley Online Library
Abstract Taxonomy Hop stunt viroid (HSVd) is the type species of the genus Hostuviroid
(family Pospiviroidae). The other species of this genus is Dahlia latent viroid, which presents …

Artificial microRNAs and synthetic trans‐acting small interfering RNAs interfere with viroid infection

A Carbonell, JA Daròs - Molecular plant pathology, 2017 - Wiley Online Library
Artificial microRNAs (amiRNAs) and synthetic trans‐acting small interfering RNAs (syn‐
tasiRNAs) are two classes of artificial small RNAs (sRNAs) engineered to silence …