Serial femtosecond crystallography

TRM Barends, B Stauch, V Cherezov… - Nature reviews Methods …, 2022 - nature.com
With the advent of X-ray free-electron lasers (XFELs), new, high-throughput serial
crystallography techniques for macromolecular structure determination have emerged …

[HTML][HTML] Recent results in time resolved serial femtosecond crystallography at XFELs

AM Orville - Current Opinion in Structural Biology, 2020 - Elsevier
Highlights•Time-resolved serial femtosecond crystallography (tr-SFX) probes structure and
function in each sample.•X-ray free electron lasers (XFELS) provide atomic and electronic …

A MHz-repetition-rate hard X-ray free-electron laser driven by a superconducting linear accelerator

W Decking, S Abeghyan, P Abramian, A Abramsky… - Nature …, 2020 - nature.com
The European XFEL is a hard X-ray free-electron laser (FEL) based on a high-electron-
energy superconducting linear accelerator. The superconducting technology allows for the …

[HTML][HTML] Megahertz serial crystallography

MO Wiedorn, D Oberthür, R Bean, R Schubert… - Nature …, 2018 - nature.com
The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of
delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of …

[HTML][HTML] Ultracompact 3D microfluidics for time-resolved structural biology

J Knoška, L Adriano, S Awel, KR Beyerlein… - Nature …, 2020 - nature.com
To advance microfluidic integration, we present the use of two-photon additive
manufacturing to fold 2D channel layouts into compact free-form 3D fluidic circuits with …

[HTML][HTML] Four-dimensional metal-organic frameworks

JD Evans, V Bon, I Senkovska, HC Lee… - Nature …, 2020 - nature.com
Recognising timescale as an adjustable dimension in porous solids provides a new
perspective to develop novel four-dimensional framework materials. The deliberate design …

X-ray free-electron lasers for the structure and dynamics of macromolecules

HN Chapman - Annual review of biochemistry, 2019 - annualreviews.org
X-ray free-electron lasers provide femtosecond-duration pulses of hard X-rays with a peak
brightness approximately one billion times greater than is available at synchrotron radiation …

Time-resolved serial femtosecond crystallography at the European XFEL

S Pandey, R Bean, T Sato, I Poudyal, J Bielecki… - Nature …, 2020 - nature.com
Abstract The European XFEL (EuXFEL) is a 3.4-km long X-ray source, which produces
femtosecond, ultrabrilliant and spatially coherent X-ray pulses at megahertz (MHz) repetition …

[HTML][HTML] Serial protein crystallography in an electron microscope

R Bücker, P Hogan-Lamarre, P Mehrabi… - Nature …, 2020 - nature.com
Serial X-ray crystallography at free-electron lasers allows to solve biomolecular structures
from sub-micron-sized crystals. However, beam time at these facilities is scarce, and …

[HTML][HTML] Megahertz single-particle imaging at the European XFEL

E Sobolev, S Zolotarev, K Giewekemeyer… - Communications …, 2020 - nature.com
The emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by
superconducting accelerator technology enables the measurement of significantly more …