Stabilizers and their interaction with formulation components in frozen and freeze-dried protein formulations
This review aims to provide an overview of the current knowledge on protein stabilization
during freezing and freeze-drying in relation to stress conditions commonly encountered …
during freezing and freeze-drying in relation to stress conditions commonly encountered …
[HTML][HTML] Understanding and controlling the molecular mechanisms of protein aggregation in mAb therapeutics
In antibody development and manufacturing, protein aggregation is a common challenge
that can lead to serious efficacy and safety issues. To mitigate this problem, it is important to …
that can lead to serious efficacy and safety issues. To mitigate this problem, it is important to …
Embedding dynamics in intrinsic physicochemical profiles of market-stage antibody-based biotherapeutics
Adequate stability, manufacturability, and safety are crucial to bringing an antibody-based
biotherapeutic to the market. Following the concept of holistic in silico developability, we …
biotherapeutic to the market. Following the concept of holistic in silico developability, we …
[HTML][HTML] Engineering stability, viscosity, and immunogenicity of antibodies by computational design
D Kuroda, K Tsumoto - Journal of Pharmaceutical Sciences, 2020 - Elsevier
In recent years, computational methods have garnered much attention in protein
engineering. A large number of computational methods have been developed to analyze the …
engineering. A large number of computational methods have been developed to analyze the …
Computational Characterization of Antibody–Excipient Interactions for Rational Excipient Selection Using the Site Identification by Ligand Competitive Saturation …
Protein therapeutics typically require a concentrated protein formulation, which can lead to
self-association and/or high viscosity due to protein–protein interaction (PPI). Excipients are …
self-association and/or high viscosity due to protein–protein interaction (PPI). Excipients are …
Flow activation energy of high-concentration monoclonal antibody solutions and protein–protein interactions influenced by NaCl and sucrose
The solution viscosity and protein–protein interactions (PPIs) as a function of temperature (4–
40° C) were measured at a series of protein concentrations for a monoclonal antibody (mAb) …
40° C) were measured at a series of protein concentrations for a monoclonal antibody (mAb) …
[HTML][HTML] The search for novel proline analogs for viscosity reduction and stabilization of highly concentrated monoclonal antibody solutions
M Prašnikar, M Proj, MB Žiberna, B Lebar… - International journal of …, 2024 - Elsevier
Administration of monoclonal antibodies (mAbs) is currently focused on subcutaneous
injection associated with increased patient adherence and reduced treatment cost, leading …
injection associated with increased patient adherence and reduced treatment cost, leading …
An experimental approach probing the conformational transitions and energy landscape of antibodies: a glimmer of hope for reviving lost therapeutic candidates using …
Understanding protein folding in different environmental conditions is fundamentally
important for predicting protein structures and developing innovative antibody formulations …
important for predicting protein structures and developing innovative antibody formulations …
Computational models for studying physical instabilities in high concentration biotherapeutic formulations
MA Blanco - MAbs, 2022 - Taylor & Francis
Computational prediction of the behavior of concentrated protein solutions is particularly
advantageous in early development stages of biotherapeutics when material availability is …
advantageous in early development stages of biotherapeutics when material availability is …
Machine learning models of antibody–excipient preferential interactions for use in computational formulation design
Preferential interactions of formulation excipients govern their impact on the stability
properties of proteins in solution. The ability to predict these interactions without the need to …
properties of proteins in solution. The ability to predict these interactions without the need to …