Targeted integration of large DNA cargoes (> 10 kb) or genomic replacements in
mammalian cells, such as human pluripotent stem cells (hPS cells), remains challenging …

Plant biotechnologists seek to modify plants through genetic reprogramming, but our ability
to precisely control gene expression in plants is still limited. Here, we review transcription …

Transcription-coupled supercoiling of DNA is a key factor in chromosome compaction and
the regulation of genetic processes in all domains of life. It has become common knowledge …

Transcription has a mechanical component, as the translocation of the transcription
machinery or RNA polymerase (RNAP) on DNA or chromatin is dynamically coupled to the …

Vinblastine is a chemotherapy agent produced by the plant Catharanthus roseus in small
quantities. Currently, vinblastine is sourced by isolation or semisynthesis. Nicotiana …

Topoisomerase I (TOP1) is an essential enzyme that relaxes DNA to prevent and dissipate
torsional stress during transcription. However, the mechanisms underlying the regulation of …

The origin and fixation of evolutionarily young genes is a fundamental question in
evolutionary biology. However, understanding the origins of newly evolved genes arising de …

Cells provide dynamic platforms for executing exogenous genetic programs in synthetic
biology, resulting in highly context-dependent circuit performance. Recent years have seen …

The field of synthetic biology and biosystems engineering increasingly acknowledges the
need for a holistic design approach that incorporates circuit-host interactions into the design …

Resource competition can be the cause of unintended coupling between co-expressed
genetic constructs. Here we report the quantification of the resource load imposed by …