A critical review: Recent advances in “digital” biomolecule detection with single copy sensitivity

H Liu, Y Lei - Biosensors and Bioelectronics, 2021 - Elsevier
Detection of a single biomolecule, ranging from nucleic acids, proteins, viruses to bacteria, is
of paramount importance in various fields including biology, environment, food and …

Next-generation rapid phenotypic antimicrobial susceptibility testing

G Reszetnik, K Hammond, S Mahshid… - Nature …, 2024 - nature.com
Slow progress towards implementation of conventional clinical bacteriology in low resource
settings and strong interest in greater speed for antimicrobial susceptibility testing (AST) …

[HTML][HTML] Mini-review: Recent advances in imaging-based rapid antibiotic susceptibility testing

D Song, Y Lei - Sensors and Actuators Reports, 2021 - Elsevier
The rapid emergence and spread of antibiotic-resistant bacteria have become a major threat
to public health. In clinics, antibiotic susceptibility testing (AST) is employed to guide …

High-Throughput Monitoring of Pathogenic Fungal Growth Using Whole Slide Imaging for Rapid Antifungal Susceptibility Assessment

D Song, H Liu, Y Huang, A Dongari-Bagtzoglou… - Analytical …, 2024 - Taylor & Francis
Invasive fungal infections are a major health threat with high morbidity and mortality,
highlighting the urgent need for rapid diagnostic tools to detect antifungal resistance …

Highly sensitive digital detection of SARS-CoV-2 nucleocapsid protein through single-molecule counting

X Dou, Z Zhang, B Liu, C Li, Y Du, F Tian - Analytical and Bioanalytical …, 2023 - Springer
Nucleocapsid protein (NP) is one of the structural proteins of SARS-CoV-2 which is stable,
well-conserved, highly immunogenic, and abundantly expressed due to the host's adaptive …

Geospatial spread of antimicrobial resistance, bacterial and fungal threats to Coronavirus Infectious Disease 2019 (COVID-19) survival, and point-of-care solutions

GJ Kost - Archives of pathology & laboratory medicine, 2021 - meridian.allenpress.com
Context.—Point-of-care testing (POCT) is inherently spatial, that is, performed where
needed, and intrinsically temporal, because it accelerates decision-making. POCT efficiency …

[HTML][HTML] Whole slide imaging is a high-throughput method to assess Candida biofilm formation

MWD Raas, TP Silva, JCO Freitas, LM Campos… - Microbiological …, 2021 - Elsevier
New strategies that enable fast and accurate visualization of Candida biofilms are necessary
to better study their structure and response to antifungals agents. Here, we applied whole …

Phase contrast image-based rapid antimicrobial susceptibility testing of bacteria in liquid culture media

X Zhang, X Wang, Y Bao, Z Shen, Y Xu, B Wang… - Sensors, 2022 - mdpi.com
Currently, the world is facing the problem of bacterial resistance, which threatens public
health, and bacterial antimicrobial susceptibility testing (AST) plays an important role in …

A whole area scanning–enabled direct-counting strategy for studying blocking efficiency in mitigating protein-solid surface binding

H Liu, Y Huang, Y Lei - Analytical and bioanalytical chemistry, 2021 - Springer
The study of protein-solid surface binding as well as blocking efficiency of blocking agents
plays an important role in the development of high-performance immunoassays. Although …

Whole slide imaging to understand Candida biofilm growth and response to antifungals

MWD Raas, TP Silva, JCO Freitas, LM Campos… - bioRxiv, 2020 - biorxiv.org
Biofilm formation is a potent factor in the pathogenesis of Candida species. The
development of new strategies that enable fast and entire visualization of Candida biofilms …