Genetic transformation technology: status and problems

KK Sharma, P Bhatnagar-Mathur, TA Thorpe - In Vitro Cellular & …, 2005 - Springer
Transfer of genes from heterologous species provides the means of selectively introducing
new traits into crop plants and expanding the gene pool beyond what has been available to …

Agrobacterium tumefaciens-mediated transformation of recalcitrant crops

MF van Wordragen, HJM Dons - Plant Molecular Biology Reporter, 1992 - Springer
The most widely used technique for the introduction of new genetic information into plant
cells is based on the natural gene transfer capacity of Agrobacterium tumefaciens. Currently …

Analysis of mannose selection used for transformation of sugar beet

M Joersbo, I Donaldson, J Kreiberg, SG Petersen… - Molecular …, 1998 - Springer
Various factors affecting mannose selection for the production of transgenic plants were
studied using Agrobacterium tumefaciens-mediated transformation of sugar beet (Beta …

The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species

I Godwin, G Todd, B Ford-Lloyd, HJ Newbury - Plant Cell Reports, 1991 - Springer
Expiants of five plant species (Allium cepa, Antirrhinum majus, Brassica campestris. Glycine
max, and Nicotiana tabacum) were co-cultivated with three Agrobacterium tumefaciens …

Genetically modified crops and hybridization with wild relatives: a UK perspective

AF Raybould, AJ Gray - Journal of Applied Ecology, 1993 - JSTOR
1. It has been suggested that genetic modification could lead to crops with enhanced
invasiveness and persistence. These new genotypes could invade natural ecosystems and …

Transgenic salt‐tolerant sugar beet (Beta vulgaris L.) constitutively expressing an Arabidopsis thaliana vacuolar Na+/H+ antiporter gene, AtNHX3, accumulates …

HUA Liu, Q Wang, M Yu, Y Zhang, Y Wu… - Plant, cell & …, 2008 - Wiley Online Library
In Arabidopsis thaliana, six vacuolar Na+/H+ antiporters (AtNHX1‐6) were identified. Among
them, AtNHX1, 2 and 5 are functional Na+/H+ antiporters with the most abundant expression …

Effective selection and regeneration of transgenic rice plants with mannose as selective agent

P Lucca, X Ye, I Potrykus - Molecular Breeding, 2001 - Springer
A new method for the selection of transgenic rice plants without the use of antibiotics or
herbicides has been developed. The phosphomannose isomerase (PMI) gene from …

A conditional marker gene allowing both positive and negative selection in plants

O Erikson, M Hertzberg, T Näsholm - Nature biotechnology, 2004 - nature.com
Selectable markers enable transgenic plants or cells to be identified after transformation.
They can be divided into positive and negative markers conferring a selective advantage or …

Activities of CaMV 35S and nos promoters in pollen: implications for field release of transgenic plants

JE Wilkinson, D Twell, K Lindsey - Journal of experimental …, 1997 - academic.oup.com
The expression of foreign genes in pollen may pose potential problems in the field release
of transgenic plants, since pollen represents a route whereby foreign genes and their …

The experimental and commercial release of transgenic crop plants

PJ Dale, JA Irwin, JA Scheffler - Plant breeding, 1993 - Wiley Online Library
With advances in recombinant DNA methods and transformation procedures, it is possible to
transfer genes into crop plants from unrelated plants, microbes and animals. Many of the …