A review on complete silk gene sequencing and de novo assembly of artificial silk

W Lu, R Shi, X Li, S Ma, D Yang, D Shang… - International Journal of …, 2024 - Elsevier
Silk, especially spider and insect silk, is a highly versatile biomaterial with potential
applications in biomedicine, materials science, and biomimetic engineering. The primary …

Phylogenomics recovers multiple origins of portable case making in caddisflies (Insecta: Trichoptera), nature's underwater architects

PB Frandsen, RW Holzenthal… - … of the Royal …, 2024 - royalsocietypublishing.org
Caddisflies (Trichoptera) are among the most diverse groups of freshwater animals with
more than 16 000 described species. They play a fundamental role in freshwater ecology …

Unraveling the genetics of underwater caddisfly silk

S Standring, J Heckenhauer, RJ Stewart, PB Frandsen - Trends in Genetics, 2025 - cell.com
Hundreds of thousands of arthropod species use silk to capture prey, build protective
structures, or anchor eggs. While most silk-producers are terrestrial, caddisflies construct …

Allelic resolution of insect and spider silk genes reveals hidden genetic diversity

PB Frandsen, S Hotaling, A Powell… - Proceedings of the …, 2023 - National Acad Sciences
Arthropod silk is vital to the evolutionary success of hundreds of thousands of species. The
primary proteins in silks are often encoded by long, repetitive gene sequences. Until …

Highly accurate long reads are crucial for realizing the potential of biodiversity genomics

S Hotaling, ER Wilcox, J Heckenhauer, RJ Stewart… - BMC genomics, 2023 - Springer
Background Generating the most contiguous, accurate genome assemblies given available
sequencing technologies is a long-standing challenge in genome science. With the rise of …

Genomic resources of aquatic Lepidoptera, Elophila obliteralis and Hyposmocoma kahamanoa, reveal similarities with Trichoptera in amino acid composition of …

J Heckenhauer, D Plotkin, JI Martinez… - G3: Genes …, 2024 - academic.oup.com
While most species of butterflies and moths (Lepidoptera) have entirely terrestrial life
histories,∼ 0.5% of the described species are known to have an aquatic larval stage. Larvae …

Chromosome segregation during spermatogenesis occurs through a unique center-kinetic mechanism in holocentric moth species

C Hockens, H Lorenzi, TT Wang, EP Lei… - PLoS …, 2024 - journals.plos.org
Precise regulation of chromosome dynamics in the germline is essential for reproductive
success across species. Yet, the mechanisms underlying meiotic chromosomal events such …

Characterization of the primary structure of the major silk gene, h-fibroin, across caddisfly (Trichoptera) suborders

J Heckenhauer, RJ Stewart, B Ríos-Touma, A Powell… - Iscience, 2023 - cell.com
Larvae of caddisflies (Trichoptera) produce silk to build various underwater structures
allowing them to exploit a wide range of aquatic environments. The silk adheres to various …

Combined CRISPR toolkits reveal the domestication landscape and function of the ultra-long and highly repetitive silk genes

W Lu, S Ma, L Sun, T Zhang, X Wang, M Feng, A Wang… - Acta Biomaterialia, 2023 - Elsevier
Highly repetitive sequences play a major structural and function role in the genome. In the
present study, we developed Cas9-assisted cloning and S MRT sequencing of long …

De Novo Long-Read Genome Assembly and Annotation of the Luna Moth (Actias luna) Fully Resolves Repeat-Rich Silk Genes

A Markee, RK Godfrey, PB Frandsen… - Genome Biology and …, 2024 - academic.oup.com
We present the first long-read de novo assembly and annotation of the luna moth (Actias
luna) and provide the full characterization of heavy chain fibroin (h-fibroin), a long and highly …