High-speed atomic force microscopy coming of age

T Ando - Nanotechnology, 2012 - iopscience.iop.org
High-speed atomic force microscopy (HS-AFM) is now materialized. It allows direct
visualization of dynamic structural changes and dynamic processes of functioning biological …

High-speed AFM and applications to biomolecular systems

T Ando, T Uchihashi, N Kodera - Annual review of biophysics, 2013 - annualreviews.org
Directly observing individual protein molecules in action at high spatiotemporal resolution
has long been a holy grail for biological science. This is because we long have had to infer …

Imaging of nucleic acids with atomic force microscopy

YL Lyubchenko, LS Shlyakhtenko, T Ando - Methods, 2011 - Elsevier
Atomic force microscopy (AFM) is a key tool of nanotechnology with great importance in
applications to DNA nanotechnology and to the recently emerging field of RNA …

[HTML][HTML] Long-tip high-speed atomic force microscopy for nanometer-scale imaging in live cells

M Shibata, T Uchihashi, T Ando, R Yasuda - Scientific reports, 2015 - nature.com
Visualization of morphological dynamics of live cells with nanometer resolution under
physiological conditions is highly desired, but challenging. It has been demonstrated that …

Dynamics of nucleosomes assessed with time-lapse high-speed atomic force microscopy

A Miyagi, T Ando, YL Lyubchenko - Biochemistry, 2011 - ACS Publications
A fundamental challenge of gene regulation is the accessibility of DNA within nucleosomes.
Recent studies performed by various techniques, including single-molecule approaches, led …

Real-time multistep asymmetrical disassembly of nucleosomes and chromatosomes visualized by high-speed atomic force microscopy

B Onoa, C Díaz-Celis, C Cañari-Chumpitaz… - ACS Central …, 2023 - ACS Publications
During replication, expression, and repair of the eukaryotic genome, cellular machinery must
access the DNA wrapped around histone proteins forming nucleosomes. These octameric …

High-speed atomic force microscopy combined with inverted optical microscopy for studying cellular events

Y Suzuki, N Sakai, A Yoshida, Y Uekusa, A Yagi… - Scientific reports, 2013 - nature.com
A hybrid atomic force microscopy (AFM)-optical fluorescence microscopy is a powerful tool
for investigating cellular morphologies and events. However, the slow data acquisition rates …

Preparation of DNA and nucleoprotein samples for AFM imaging

YL Lyubchenko - Micron, 2011 - Elsevier
Sample preparation techniques allowing reliable and reproducible imaging of DNA with
various structures, topologies and complexes with proteins are reviewed. The major …

State-of-the-art high-speed atomic force microscopy for investigation of single-molecular dynamics of proteins

A Rajendran, M Endo, H Sugiyama - Chemical reviews, 2014 - ACS Publications
Proteins are probably the most important biomacromolecules and the building blocks of life.
They regulate a large number of diverse biological functions. Protein function depends …

[图书][B] Handbook of clinical nanomedicine: nanoparticles, imaging, therapy, and clinical applications

R Bawa, GF Audette, I Rubinstein - 2016 - books.google.com
This handbook (55 chapters) provides a comprehensive roadmap of basic research in
nanomedicine as well as clinical applications. However, unlike other texts in nanomedicine …