Ribozymes can catalyze a large variety of different reactions, but very few mechanistic
aspects are understood in detail. 1-9 It is evident that RNAs are always associated with …

Background The accompanying article (AY Mulkidjanian, Biology Direct 4: 26) puts forward
a detailed hypothesis on the role of zinc sulfide (ZnS) in the origin of life on Earth. The …

The acidity constants for (N3) H of the uridine-type ligands (U) 5-fluorouridine, 5-chloro-2′-
deoxyuridine, uridine, and thymidine (2′-deoxy-5-methyluridine) and the stability constants …

A decamer duplex model of Domain II of the hammerhead ribozyme was synthesized with [8-
13C-1, 7, NH2-15N3]-guanosine at the known metal binding site, G10. 1 and, for …

By employing classical molecular dynamics, correlation analysis of coupling between slow
and fast dynamical modes, and free energy (umbrella) sampling using classical as well as …

Background Hammerhead ribozymes are RNA-based molecules which bind and cleave
other RNAs specifically. As such they have potential as laboratory reagents, diagnostics and …

The crystal structure of subtype-B HIV-1 genomic RNA Dimerization Initiation Site duplex
revealed chain cleavage at a specific position resulting in 3′-phosphate and 5′-hydroxyl …

Here we present the in vitro selection of a novel ribozyme specific for Zn2+-dependent
catalysis on hydrolysis of a phosphorothiolate thiolester bond. The ribozyme, called the …

Abstract The modified nucleosides 4-thio-2′-deoxyuridine (s 4 dU) and 4-thio-2′-
deoxythymidine (s 4 dT) are incorporated into dinucleosides, and s 4 dT is incorporated into …

Hammerhead ribozymes in crystals change conformation in response to deprotonation of
the nucleophilic 2′ OH, thereby aligning the hydroxyl for in-line displacement at the scissile …