Native chemical ligation (NCL) provides a highly efficient and robust means to
chemoselectively link unprotected peptide and protein segments to generate proteins. The …

Protein semisynthesis—defined herein as the assembly of a protein from a combination of
synthetic and recombinant fragments—is a burgeoning field of chemical biology that has …

The chemical synthesis of homogeneously modified histones is a powerful approach to
quantitatively decipher how post-translational modifications (PTMs) modulate epigenetic …

The Polycomb repressive complexes PRC1, PRC2, and PR-DUB repress target genes by
modifying their chromatin. In Drosophila, PRC1 compacts chromatin and monoubiquitinates …

Nucleosomes and chromatin control eukaryotic genome accessibility and thereby regulate
DNA processes, including transcription, replication, and repair. Conformational dynamics …

Pioneer transcription factors (pTFs) bind to target sites within compact chromatin, initiating
chromatin remodeling and controlling the recruitment of downstream factors. The …

Histone ubiquitination affects the structure and function of nucleosomes through tightly
regulated dynamic reversible processes. The efficient preparation of ubiquitinated histones …

Ferricyanide-promoted oxidative activation of N-acylated α-aminothioacids for amide bond
formation with α-aminonitriles was recently shown to be a plausible pathway for prebiotic …

One‐pot multiple peptide ligation is a key technology to improve the efficiency of chemical
protein synthesis. One‐pot repetitive peptide ligation requires a cycle of three steps: peptide …

The SUV39 class of methyltransferase enzymes deposits histone H3 lysine 9 di-and
trimethylation (H3K9me2/3), the hallmark of constitutive heterochromatin. How these …