Mutations, the fuel of evolution, are first manifested as rare DNA changes within a population
of cells. Although next-generation sequencing (NGS) technologies have revolutionized the …

High-throughput sequencing of B-cell immunoglobulin repertoires is increasingly being
applied to gain insights into the adaptive immune response in healthy individuals and in …

The cell is the unit of life for all organisms, and all cells are certainly not the same. So the
technology to generate transcription expression or genomic DNA profiles from single cells is …

Validating the sampling depth and reducing sequencing errors are critical for studies of viral
populations using next-generation sequencing (NGS). We previously described the use of …

Indexing individual template molecules with a unique identifier (UID) before PCR and deep
sequencing is promising for detecting low frequency mutations, as true mutations could be …

Next-generation sequencing (NGS) technologies have come of age as preferred
technologies for screening of genomic variants of pathologic and therapeutic potential …

Motivation Low-frequency DNA mutations are often confounded with technical artifacts from
sample preparation and sequencing. With unique molecular identifiers (UMIs), most of the …

Nucleotide incorporation and lacZ-based forward mutation assays have been widely used to
determine the accuracy of reverse transcriptases (RTs) in RNA-dependent DNA …

In this paper, we present a toolset and related resources for rapid identification of viruses
and microorganisms from short-read or long-read sequencing data. We present fastv as an …

Next generation sequencing (NGS)/deep sequencing has become an important tool in the
study of viruses. The use of unique molecular identifiers (UMI) can overcome the limitations …