Genetic regulation and manipulation of nicotine biosynthesis in tobacco: strategies to eliminate addictive alkaloids

T Shoji, T Hashimoto, K Saito - Journal of Experimental Botany, 2024 - academic.oup.com
Tobacco (Nicotiana tabacum L.) is a widely cultivated crop of the genus Nicotiana. Due to
the highly addictive nature of tobacco products, tobacco smoking remains the leading cause …

Metabolic engineering for enhanced oil in biomass

T Vanhercke, JM Dyer, RT Mullen, A Kilaru… - Progress in lipid …, 2019 - Elsevier
The world is hungry for energy. Plant oils in the form of triacylglycerol (TAG) are one of the
most reduced storage forms of carbon found in nature and hence represent an excellent …

[HTML][HTML] The family of berberine bridge enzyme-like enzymes: A treasure-trove of oxidative reactions

B Daniel, B Konrad, M Toplak, M Lahham… - Archives of Biochemistry …, 2017 - Elsevier
Biological oxidations form the basis of life on earth by utilizing organic compounds as
electron donors to drive the generation of metabolic energy carriers, such as ATP. Oxidative …

[HTML][HTML] Nicotine‐free, nontransgenic tobacco (Nicotiana tabacum l.) edited by CRISPR‐Cas9

J Schachtsiek, F Stehle - Plant biotechnology journal, 2019 - ncbi.nlm.nih.gov
Worldwide, approximately 1.1 billion people are smokers and more than 7 million people die
from the negative effects of smoking every year (WHO report, 2017). One of the main natural …

Agronomic practices affecting nicotine concentration in flue‐cured tobacco: A review

JB Henry, MC Vann, RS Lewis - Agronomy Journal, 2019 - Wiley Online Library
Proposed regulations mandating lower nicotine concentrations in tobacco (Nicotiana
tabacum L.) products will likely require changes in tobacco production to reduce nicotine …

Natural and induced variations in transcriptional regulator genes result in low‐nicotine phenotypes in tobacco

T Shoji, K Moriyama, N Sierro, S Ouadi… - The Plant …, 2022 - Wiley Online Library
In tobacco, the homologous ETHYLENE RESPONSE FACTOR (ERF) transcription factors
ERF199 and ERF189 coordinate the transcription of multiple metabolic genes involved in …

Genetic manipulation of transcriptional regulators alters nicotine biosynthesis in tobacco

S Hayashi, M Watanabe, M Kobayashi… - Plant and Cell …, 2020 - academic.oup.com
The toxic alkaloid nicotine is produced in the roots of Nicotiana species and primarily
accumulates in leaves as a specialized metabolite. A series of metabolic and transport …

Metabolization and sequestration of plant specialized metabolites in insect herbivores: Current and emerging approaches

AM Jeckel, F Beran, T Züst, G Younkin… - Frontiers in …, 2022 - frontiersin.org
Herbivorous insects encounter diverse plant specialized metabolites (PSMs) in their diet,
that have deterrent, anti-nutritional, or toxic properties. Understanding how they cope with …

Nicotine Biosynthesis in Nicotiana: A Metabolic Overview

FF Zenkner, M Margis-Pinheiro… - Tobacco …, 2019 - meridian.allenpress.com
Alkaloids are important compounds found in Nicotiana plants, essential in plant defense
against herbivores. The main alkaloid of Nicotiana tabacum, nicotine, is produced in roots …

An improved Nicotiana benthamiana bioproduction chassis provides novel insights into nicotine biosynthesis

K Vollheyde, QM Dudley, T Yang, MT Oz… - New …, 2023 - Wiley Online Library
The model plant Nicotiana benthamiana is an increasingly attractive organism for the
production of high‐value, biologically active molecules. However, N. benthamiana …